仔猪腹泻大肠杆菌流行株鉴定及毒力与耐药性分析

doi:10.16431/j.cnki.1671-7236.2017.04.040

Abstract

Abstract:

In order to clarify the pathogenic characteristics and drug-resistant spectrum of a newborn piglet diarrhea outbroke recently in a large-scale pig farm of Henan province, the study was carried out by the isolation of intestinal pathogenic Escherichia coli and system identification. Escherichia coli strains were isolated from the faeces of diarrhea piglets. 13 virulent genes were tested by PCR. The antibiotic susceptibility of the strains was determined. The results showed that the positive rate came up with 34.8% for EAST1 gene. All of the strains were multiple-drug resistance and resistant to at least 5 types of antibiotics. 75.3% strains were resistant to more than 9 types of antibiotics. All of the strains were resistant to ampicillin, piperacillin, ciprofloxacin, levofloxacin and moxifloxacin. The result of the phylogenetic background showed that 21.7% E.coli isolates belonged to group B2 and D which were pathogenic. According to the synthesis analysis of virulence factors, resistance and phylogenetic background of the strains isolated from the diarrhea piglets, proper clinical treatment and prevention and control measure could be provided.

Key words: newborn piglet; diarrhea; Escherichia coli; virulence gene; drug resistance

CLC Number:

S858.28

LIU Zhe, SUN Yang, JI Xue, GUO Xue-jun, LIU Jun, ZHU Ling-wei, ZHOU Wei, FENG Shu-zhang. Identification，Virulence and Drug Resistance Analysis of Escherichia coli from Diarrhea Piglets[J]. , 2017, 44(4): 1226-1232.

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Identification，Virulence and Drug Resistance Analysis of Escherichia coli from Diarrhea Piglets

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