复方中药偶蹄康对奶牛口蹄疫疫苗抗体水平、血清细胞因子及生化指标的影响

doi:10.16431/j.cnki.1671-7236.2017.04.039

Abstract

Abstract:

The experiment was aimed to study the effects of herbal compound Outikang on antibody titer of foot and mouth disease vaccine type O,type A and type Asia Ⅰ,serum levels of ten cytokines and biochemical indices. 200 healthy adult cows were averagely divided into drug group and blank control group. Vaccinating the cows with FMD triplex vaccine when Outikang was given to the cows of drug group for 7 d running and then got the blood of all the cows on 0, 14, 28 and 56 d, respectively. Then blood biochemical examination, vaccine effectiveness and serum levels of cytokines were performed. The results showed that there were no significant differences in antibody titer of FMDV between blank control group and drug group on 0, 28 and 56 d after vaccination (P>0.05), while on 14 d the effect of Outikang on antibody titer of FMDV reached extremely significant level (P<0.01). Compared with blank control group, the contents of T-Bil in drug group was significantly decreased on 0 and 28 d (P<0.05), while it was extremely significantly decreased on 56 d (P<0.01).Compared with blank control group, the contents of Glu had significant difference on 28 and 56 d (P<0.05).The contents of P had extremely significant difference on 0 d (P<0.01), which was within the normal range. On 14 d after vaccination, the content of CD4 significantly increased in the drug group comparing to those in blank control group (P<0.05). On 28 d after vaccination, the content of CD4, IL-2, IL-6, IL-10, IL-12, IL-18 and IFN-γ extremely significantly increased in the drug group comparing to those in blank control group (P<0.01). On 56 d after vaccination, the contents of IL-6 and IL-12 extremely significantly increased in the drug group comparing to those in blank control group (P<0.01), while the content of CD4, IL-2, IL-4 and IL-18 significantly increased (P<0.05). Therefore, Outikang could significantly rise the antibody titer of foot and mouth disease vaccine type O, type A and type Asia Ⅰ.While it had no damage to the liver and kidney functions and had cholagogue effect, and it could enhance cellular immunity and humoral immunity function obviously.

Key words: Outikang; cows; foot and mouth disease; antibody titer; cytokines; serum biochemical indices

CLC Number:

S858.23

LIU Yan, PENG Wen-jing, XIN Rui-hua, ZHENG Ji-fang, LIANG Ge, LUO Yong-jiang. Effect of Herbal Compound Outikang on Antibody Titer of Foot and Mouth Disease Vaccine，Cytokines and Biochemical Indices in Cows[J]. , 2017, 44(4): 1219-1225.

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Effect of Herbal Compound Outikang on Antibody Titer of Foot and Mouth Disease Vaccine，Cytokines and Biochemical Indices in Cows

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