lncLER在卢氏绿壳蛋鸡肝脏中表达调控的研究

doi:10.16431/j.cnki.1671-7236.2017.04.005

Abstract

Abstract:

Long noncording RNA (lncRNA) played an important regulating role in many biological processes.The lncRNA expression profile of liver of pre-laying hens (20 weeks old) and peak-laying hens (30 weeks old) in Lushi Green-shelled-egg chicken were obtained by lncRNA-seq. A lncRNA, which was named lncLER and expressed highly in the peak-laying hens was singled out. To investigate the expression characteristics of lncLER in liver of chicken,reverse-transcript PCR and Real-time fluorescence quantitative PCR were used to analyze the temporal expression profile of lncLER. Both individual chicken and primary hepatocytes were used to look into the effects of estradiol on expression of the lncLER in vivo and in vitro. The results showed that lncLER was expressed differently in various tissues and it's expression level was higher in the liver of peak-laying hens. The expression of lncLER, both in individual chicken and in primary hepatocytes,was up-regulated by estradiol.This research paved the way for further studying on the biological functions of lncLER in chicken egg production.

Key words: lncRNA; Lushi Green-shelled-egg chicken; estrogen; liver; expression and regulation

CLC Number:

Q786

GU Zhen-zhen, LI Hong, LIU Yi, TAN Wen-bo, REN Jun-xiao, LI Yan-min, LI Zhuan-jian, TIAN Ya-dong, LIU Xiao-jun. The Expression and Regulation of lncLER in Liver of Lushi Green-shelled-egg Chicken[J]. , 2017, 44(4): 973-979.

References

[1] Mercer T R,Dinger M E,Mattick J S. Long non-coding RNAs:Insights into functions[J]. Nature Reviews Genetics, 2009,10(3):155-159.
[2] Clark M B,Mattick J S. Long noncoding RNAs in cell biology[J]. Seminars in Cell & Developmental Biology,2011,22(4):366-376.
[3] Zhang J,Cui X,Shen Y,et al. Distinct expression profiles of lncRNAs between brown adipose tissue and skeletal muscle[J]. Biochemical & Biophysical Research Communications,2014,443(3):1028-1034.
[4] Sun L,Goff L A,Trapnell C,et al. Long noncoding RNAs regulate adipogenesis[J]. Proceedings of the National Academy of Sciences of the United States of America,2013,110(9):3387-3392.
[5] Li P,Ruan X,Yang L,et al. A liver-enriched long non-coding RNA,lncLSTR,regulates systemic lipid metabolism in mice[J]. Cell Metabolism,2015,21(3):455-467.
[6] 郑竹清,杜琛,付绍印,等.山羊肌内脂肪细胞lncRNA鉴别及特征分析[J].畜牧兽医学报,2014,45(12):1924-1931.
[7] Schneider W J,Osanger A,Waclawek M,et al. Oocyte growth in the chicken:Receptors and more[J]. Biological Chemistry,1998,379(8-9):965-971.
[8] Li H,Wang T,Xu C,et al. Transcriptome profile of liver at different physiological stages reveals potential mode for lipid metabolism in laying hens[J]. Bmc Genomics,2015,16(1):1-13.
[9] Schmittgen T D,Livak K J. Analyzing Real-time PCR data by the comparative C(T) method[J]. Nature Protocols,2008,3(6):1101-1108.
[10] O'Hea E K,Leveille G A. Lipid biosynthesis and transport in the domestic chick (Gallus domesticus)[J]. Comparative Biochemistry & Physiology,1969,30(1):149-159.
[11] 尹靖东,齐广海.家禽脂类代谢调控机理的研究进展[J].动物营养学报,2000,12(2):1-7.
[12] Saarela J,Jung G,Hermann M,et al. The patatin-like lipase family in Gallus gallus[J]. Bmc Genomics,2008,9(1):1-15.
[13] Walzem R L,Davis P A,Hansen R J. Overfeeding increases very low density lipoprotein diameter and causes the appearance of a unique lipoprotein particle in association with failed yolk deposition[J]. Journal of Lipid Research,1994,35(8):1354-1366.
[14] Walzem R L,Hansen R J,Williams D L,et al. Estrogen induction of VLDLy assembly in egg-laying hens[J].Journal of Nutrition,1999,129(2S Suppl):467S-472S.
[15] Denslow N D,Chow M C,Kroll K J,et al. Vitellogenin as a biomarker of exposure for estrogen or estrogen mimics[J]. Ecotoxicology,1999,8(5):385-398.
[16] Matozzo V,Gagné F,Marin M G,et al. Vitellogenin as a biomarker of exposure to estrogenic compounds in aquatic invertebrates:A review[J]. Environment International,2008,34(4):531-545.
[17] Zhang J,Cui X,Shen Y,et al. Distinct expression profiles of lncRNAs between brown adipose tissue and skeletal muscle[J]. Biochemical & Biophysical Research Communications,2014,443(3):1028-1034.
[18] Ørom U A,Derrien T,Beringer M,et al. Long noncoding RNAs with enhancer-like function in human cells[J]. Cell,2010,143(1):46-58.
[19] Morlando M,Ballarino M,Fatica A. Long non-coding RNAs:New players in hematopoiesis and leukemia[J]. Frontiers in Medicine,2015,2:23.
[20] Vosper H,Patel L,Graham T L,et al. The peroxisome proliferator-activated receptor delta promotes lipid accumulation in human macrophages[J]. Journal of Biological Chemistry,2001,276(47):44258-44265.
[21] Cabili M N,Trapnell C,Goff L,et al. Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses[J]. Genes & Development,2011,25(18):1915-1927.
[22] Mercer T R,Dinger M E,Sunkin S M,et al. Specific expression of long noncoding RNAs in the mouse brain[J]. Proceedings of the National Academy of Sciences,2008,105(2):716-721.

The Expression and Regulation of lncLER in Liver of Lushi Green-shelled-egg Chicken

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Jie, CHEN Chuwen, ZHAO Ruipeng, LIU Yuan, LI Zhixiong. Research Progress on Long Non-coding RNA of Muscle Development in Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2427-2438.
[2]	XU Jiahui, XIE Jiaxin, JIANG Chengkun, ZHANG Sen, YAO Hua, ZHANG Hua. Application of Three-dimensional Reconstruction and 3D Printing Technology in Beagle Dog Liver Model Construction [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2130-2138.
[3]	WANG Yunlu, MENG Zhaoyi, YAO Yilong, GUO Min, NIU Jiaqaing, SUOLANG Sizhu, XU Yefen. Screening and Targeting Verification of lncRNA of "Sponge Adsorption" bta-miR-146a in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 859-869.
[4]	LEI Qi, REN Jie, KANG Jie. Effects of Scutellaria baicalensis georgi on Antioxidant Capacity,Sex Hormone Levels and mRNA Expression of ERα in Female Mice Exposed to ZEA [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 989-997.
[5]	WU Tao, QI Yunxia, LIU Wei, ZHU Honglong, HUANG Dongwei, DING Haisheng, ZHAO Huiling, CHENG Guanglong, ZHAO Xiaowei. Effect of Colostrum and Milk Feeding on Hepatic Proteins of Calves Based on Proteomics Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 37-45.
[6]	GAO Xiaomin, ZHOU Shujian, CHEN Chen, JIN Jing, HU Cai, ZHANG Chen, ZUO Qisheng, ZHANG Yani, CHEN Guohong, LI Bichun. Regulation of STAT1 and Histone Acetylation Modification on lncRNA-BMP4 Transcription in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3321-3332.
[7]	YU Peng, NIU Xiaoyu, DONG Ling, LU Mengqi, CHEN Yanhong, LI Fan, SONG Hui. Research Progress on the Role of lncRNA in Porcine Abortion-related Virus Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3559-3568.
[8]	ZHU Xuedan, PAN Xueqing, MA Junyan, ZHOU Lele, ZHANG Shouquan. Research Progress on the Effects of Prostaglandin F_2α and Its Analogues on the Reproductive Performance of Mammal [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3072-3082.
[9]	CHEN Shiyu, CUI Shuai, JIANG Yajun, XIN Ting, WANG Yang, HAO Yuxin, HUANG Jianxin, GUO Xiaoyu, ZHU Hongfei, WU Jiajun, JIA Hong. lncRNA-mRNA Analysis of Toll-like Receptor Signaling in Porcine Peripheral Blood Lymphocytes After African Swine Fever Virus Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2677-2687.
[10]	SHEN Xingling, DING Kangning, WANG Youcong, ZHANG Yian, LIU Yilei, LIU Hanxiao, TANG Luping, HE Yongming. Study on the Mechanism of Polygonum chinense L. Alleviating Liver Injury Induced by Salmonella Typhimurium in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2768-2777.
[11]	XUE Qian, LI Guohui, XING Weijie, ZHOU Chenghao, ZHANG Huiyong, YIN Jianmei, ZHU Yunfen, CAO Yuxia, SU Yijun, HAN Wei. Screening of lncRNA Related to Inbreeding Depression of Reproduction in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2176-2185.
[12]	AN Zhixia, PAN Yangyang, FAN Biyue, MA Xiaoyan, YE Dehe, ZHANG Zhijie, QIU Shantong, YAO Yale, WANG Meng. Study on the Effect of Forsythia suspensa Leaf Extract on Enrofloxacin-induced Liver Injury Through AMPK Based on Network Pharmacology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2347-2361.
[13]	SU Xiaohui, HE Haiying, TUERXUNAYI·Yalikun, WANG Qiong, MA Haiyu, LIU Lingling, LIU Wujun. Identification and Analysis of Differentially Expressed lncRNA of Tail Fat in the Second Generation of Crossbred from Wild Argali×Bashibai Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1796-1805.
[14]	WANG Dong, LIANG Jiayue, WANG Xiaobing, FENG Juan, ZHANG Ru, XIA Huiyan, XIAO Hongmei. Research Progress of Non-coding RNA in the Development of Hair Follicles of Cashmere Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1817-1827.
[15]	SUN Qingyun, FAN Xiang, XING Ya, ZHAO Minmeng, LIU Long, GENG Tuoyu, GONG Daoqing. Study on the Relationship Between OTUD7A Gene and Goose Fatty Liver Formation [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1295-1303.