新疆猪源沙门氏菌耐药性及耐药基因检测

doi:10.16431/j.cnki.1671-7236.2017.03.038

Abstract

Abstract:

The objective of this study was to investigate the resistance of Salmonella and prevalence of resistance genes isolated from a pig farm in Xinjiang, and their coexistence with the major β-lactamases, 16S rRNA methylation enzyme genes and PMQR. The minimum inhibitory concentrations of Salmonella isolated from the pig farms were determined by agar dilution method, PCR was used to detect bla_TEM, bla_CMY-2, bla_CTX-M, bla_LAP-1, bla_KPC, bla_OXAand bla_SHV genes, 16S rRNA methylation enzyme genes armA and rmtB, and PMQR including qnrA, qnrB, qnrC, qnrD, qnrS, qepA, oqxA, oqxB and aac(6')-Ib genes. The positive strains were performed by using DNA sequencing to determine the purpose of the belt. The result showed that resistant rate of Salmonella isolates from swine were highest to ciprofloxacin and apramycin sulfate (77.9%, 102/131). Resistant mainly based on 8 kinds of drug resistance (29.0%, 38/131), 11 kinds of resistance genes were detection, the coexistence of different genotypes had 24 types. The main types were bla_TEM+bla_OXA+qnrS+aac(6')-Ib-cr+oqxA+oqxB (27.6%, 32/116), bla_TEM+bla_OXA+qnrS+oqxA+oqxB (24.1%, 28/116) and bla_TEM+qnrS (18.0%, 21/116). The Salmonella isolated from the farm had phenomenon seriously resistance, it coexisted with the main β-lactamase, 16S rRNA methylation enzyme genes and PMQR factors. The result suggested that it should strengthen monitoring to the β-lactamase enzymes, 16S rRNA methylation enzyme genes and PMQR factors.

Key words: Salmonella of swine; resistance; resistance genes; detection

CLC Number:

S852.61

JIANG Ping, GUAN Ru-fei, XIA Li-ning, LIN Ya-jun. Detection of Resistance and Resistance Genes of Salmonella from Swine in Xinjiang[J]. , 2017, 44(3): 896-903.

References

[1] Liu Y Y, Wang Y, Walsh T R, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China:A microbiological and molecular biological study[J]. Lancet Infect Dis, 2016,16(2):161-168.
[2] 黄凯,陈素娟,黄骏,等. 动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定[J]. 中国畜牧兽医,2015,42(2):459-466.
[3] 杜雄伟,李叶,王晓辉. 沙门氏菌耐药机制的研究进展[J]. 江苏农业科学,2010,6:487-490.
[4] 罗淑华, 黄鹏飞, 李燕, 等. 2010~2011年深圳市光明新区沙门氏菌流行特征及药敏分析[J]. 热带医学杂志, 2012,12(11):1370-1371.
[5] Shanmugasamy M, Velayutham T, Rajeswar J. InvA gene specific PCR for detection of Salmonella from boilers[J]. Vet World, 2011, 4(12):562-564.
[6] 李琳.沙门氏菌环丙沙星耐药性诱导及其耐药分子机制研究[D].北京:中国农业大学,2008.
[7] Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; Twentieth informational supplement[S]. CLSI documents M100-S22. CLSI, 2015.
[8] Monstein H J, Ostholm-Balkhed A, Nilsson M V, et al. Multiplex PCR amplification assay for the detection of bla_SHV, bla_TEM and bla_CTX-M genes in Enterobacteriaceae[J]. APMIS, 2007, 115(12):1400-1408.
[9] Poirel L, Cattoir V, Soares A, et al. Novel Ambler class A beta-lactamase LAP-1 and its association with the plasmid-mediated quinolone resistance determinant QnrS1[J]. Antimicrobial Agents and Chemotherapy, 2007, 51(2):631-637.
[10] Xia L N, Tao X Q, Shen J Z, et al. A survey of β-lactamase and 16S rRNA methylase genes among fluoroquinolone-resistant Escherichia coli isolates and their horizontal transmission in Shandong, China[J]. Foodborne Pathog Dis, 2011,8(12):1241-1248.
[11] Lim K T, Yeo C C, Yasin R M, et al. Characterization of multidrug-resistant and extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains from Malaysian hospitals[J]. Med Microbiol, 2009,58(11):1463-1469.
[12] Guerra B, Helmuth R, Thomas K, et al. Plasmid-mediated quinolone resistance determinants in Salmonella spp. isolates from reptiles in Germany[J]. The Journal of Antimicrobial Chemotherapy, 2010,65(9):2043-2045.
[13] Gay K, Robicsek A, Strahilevitz J, et al. Plasmid-mediated quinolone resistance in non-Typhi serotypes of Salmonella enterica[J]. Clinical Infectious Diseases, 2006,43(3):297-304.
[14] Wang M, Guo Q, Xu X, et al. New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis[J]. Antimicrob Agents Chemother, 2009,53(5):1892-1897.
[15] Cavaco L M, Hasman H, Xia S, et al. qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin[J]. Antimicrob Agents Chemother, 2009,53(2):603-608.
[16] Rodríguez-Martínez J M, Díaz de Alba P, Briales A, et al.Contribution of oqxAB efflux pumps to quinolone resistance in extended-spectrum-β-lactamase-producing Klebsiella pneumoniae[J]. The Journal of Antimicrobial Chemotherapy, 2013,68(1):68-73.
[17] 夏利宁.不同来源喹诺酮耐药大肠杆菌耐药基因的流行性调查及其传播机制研究[D].北京:中国农业大学,2010.
[18] Park C H, Robicsek A, Jacoby G A, et al. Prevalence in the United States of aac(6')-Ib-cr encoding a ciprofloxacin-modifying enzyme[J]. Antimicrobial Agents And Chemotherapy, 2006,50(11):3953-3955.
[19] Lestari S I, Han F, Wang F, et al. Prevalence and antimicrobialresistance of Salmonella serovars in conventional and organicchickens from Louisiana retail stores[J]. Food Prot, 2009,72:1165-1172.
[20] 张晓丹,高丽丽,胡家卿,等. 猪源携带质粒介导喹诺酮类耐药基因大肠杆菌气源性传播的研究[J]. 中国畜牧兽医,2015,42(7):1842-1850.
[21] 林居纯,覃春红,赖婧,等. 食品动物源沙门菌的分离鉴定及耐药性检测[J]. 中国兽医杂志,2012,48(12):63-66.
[22] 狄文婷,杜雄伟,吴静,等. 猪源沙门氏菌的分离与耐药性分析[J]. 江苏农业科学,2014,42(10):278-280.
[23] 张玮. 安徽省部分猪场生猪沙门氏菌带菌情况与耐药性研究[D]. 合肥:安徽农业大学, 2010.
[24] 金文杰,秦爱建,郑志明,等. 禽致病性大肠杆菌中四种抗氨基糖苷类药物耐药基因的分子流行病学调查[J]. 中国预防兽医学报,2007,29(5):402-404.
[25] 曹正花,谭艾娟,吕世明,等. 贵州省猪源沙门氏菌对β-内酰胺类药耐药性及耐药基因分析[J]. 中国畜牧兽医,2016,43(7):1737-1742.
[26] 南海辰,底丽娜,夏利宁. 新疆多源喹诺酮类耐药大肠杆菌耐药基因检测及分析[J]. 中国农业科学,2014,47(20):4096-4108.
[27] 马婧嘉,施春雷,李可,等. 沙门氏菌耐药谱及质粒耐药基因的筛查[J]. 中国食品学报,2014,14(4):184-190.
[28] 林居纯,覃春红,赖婧,等. 食品动物源沙门氏菌质粒介导喹诺酮类耐药基因的检测与分析[J]. 畜牧兽医学报,2012,43(5):803-809.
[29] 李德喜, 刘建华, 张素梅, 等. 猪源大肠杆菌质粒和染色体介导的喹诺酮类药的耐药机制[J]. 中国兽医学报, 2011,31(9):1262-1265.

Detection of Resistance and Resistance Genes of Salmonella from Swine in Xinjiang

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