上海地区屠宰场猪红斑丹毒丝菌的分离鉴定

doi:10.16431/j.cnki.1671-7236.2017.01.032

Abstract

Abstract:

In order to comprehend the status of Erysipelothrix rhusiopathiae carried in swinery in Shanghai area, samples of pigs were collected from some standard abattoirs in Shanghai area, including nasal swab, lung, tonsil and mandibular lymph nodes. Strains were isolated from these samples for detecting the Erysipelothrix rhusiopathiae. Several kinds of methods were used, such as VITEK 2 Compact system and VITEK MS system. A pair of primers was designed to amplify the 16S rRNA gene. 16 PCR products selected randomly were sequenced and 16S rRNA homology analysis was conducted. As a result, strains were isolated from 49.07% (105/214) samples and were identified as Erysipelothrix rhusiopathiae.16S rRNA homology in strains of isolation and reference were 99.6% to 100.0%. The result indicated that the situation was very severe for so many carriers of Erysipelothrix rhusiopathiae in health group in nursery.

Key words: abattoirs; Erysipelothrix rhusiopathiae; isolation and identification

CLC Number:

S858.28

XU Feng, WANG Xiao-xu, ZHANG Wei-yi, NING Kun, QI Xin-yong, SHEN Li-ping. Isolation and Identification of Erysipelothrix rhusiopathiae from Abattoirs in Shanghai Area[J]. , 2017, 44(1): 227-235.

References

[1] Ding Y, Zhu D M, Zhang J M, et al. Virulence determinants, antimicrobial susceptibility, and molecular profiles of Erysipelothrix rhusiopathiae strains isolated from China[J].Emerging Microbes and Infections,2015, 4(11):69.
[2] Melero M, Lirola L, Guerri C, et al. Fluorescent microbead based immunoassay for anti-Erysipelothrix rhusiopathiae antibody detection in cetaceans[J].Diseases of Aquatic Organisms, 2016, 117(3):237-243.
[3] Susan K, Trent B, Marsha B, et al. Erysipelothrix rhusiopathiae associated with recent widespread muskox mortalities in the Canadian Arctic[J].Canadian Veterinary Journal, 2015, 56(6):560-563.
[4] Lee J J, Kim D H, Lim J J, et al. Characterization and identification of Erysipelothrix rhusiopathiae isolated from an unnatural host, a cat, with a clinical manifestation of depression[J].Journal of Veterinary Medical Science,2011, 73(2):149-154.
[5] Asim A K, Alexander H, Ben M, et al. Nonspecific Erysipelothrix rhusiopathiae bacteremia in a patient with subclinical alcoholic liver disease[J]. Case Reports in Infectious Diseases, 2013, 2013:474593.
[6] Denes E, Camilleri Y, Fiorenza F, et al. First case of osteomyelitis due to Erysipelothrix rhusiopathiae:Pubic osteomyelitis in a gored farmer[J]. International Journal of Infectious Diseases, 2015, 30:133-134.
[7] Hua P, Liu J L,Tao J, et al. Erysipelothrix rhusiopathiae-induced aortic valve endocarditis:Case report and literature review[J]. International Journal of Clinical and Experimental Medicine, 2015, 8(1):730-736.
[8] Fang S, Yohsuke O, Akiyuki S, et al. Characterization and identification of a novel candidate vaccine protein through systematic analysis of extracellular proteins of Erysipelothrix rhusiopathiae[J]. Infection and Immunity,2013,81(12):4333-4340.
[9] 陆萍,黄晓慧,李春芬,等.安徽部分地区猪丹毒杆菌的分离鉴定及生物学特性研究[J].微生物学通报, 2014,41(9):1822-1828.
[10] Ho T, Hiroko S, Akihiro T, et al. Characterization of Erysipelothrix rhusiopathiae strains isolated from recent swine erysipelas outbreaks in Japan[J].Journal of Veterinary Medical Science, 2012,74(7):949-953.
[11] 徐雨露,林双喜.猪丹毒流行情况和防治措施[J].福建畜牧兽医,2013,35(4):67-68.
[12] 王洪光,汤德元,曾智勇,等.规模化猪场主要细菌性疫病的流行病学调查[J].畜牧与兽医,2014,46(9):98-100.
[13] 曾文斌,刘悦欣,王萍,等.急性死亡猪中猪丹毒杆菌的分离鉴定[J].中国畜牧兽医,2015,42(3):753-756.
[14] Zou Y, Zhu X M, Muhammad H, et al. Characterization of Erysipelothrix rhusiopathiae strains isolated from acute swine erysipelas outbreaks in Eastern China[J]. Journal of Veterinary Medical Science, 2015,77(6):653-660.
[15] 罗新民.生猪屠宰场猪丹毒杆菌污染情况调查[J].中国动物检疫,2008,25(2):34.
[16] Yohsuke O, TadasukeO, Fang S, et al. The Genome of Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, reveals new insights into the evolution of firmicutes and the organism's intracellular adaptations[J]. Journal of Bacteriology, 2011, 193(12):2959-2971.
[17] Amy H, Li Y F, Jiang J W, et al. Complete genome assembly and characterization of an outbreak strain of the causative agent of swine erysipelas——Erysipelothrix rhusiopathiae SY1027[J]. BMC Microbiology,2014, 14:176.
[18] 陈明,李健,廖红敏,等.一株小白鼠血源病原菌的分离、鉴定及生物学特性研究[J].安徽农业科学,2011,39(18):11114-11118.
[19] NY/T 566-2002猪丹毒诊断技术[S].
[20] 杨正时,房海.人及动物病原细菌学[M].石家庄:河北科学技术出版社,2003.
[21] Bender J S, Irwin C K, Shen H G, et al. Erysipelothrix spp. genotypes, serotypes, and surface protective antigen types associated with abattoir condemnations[J].Journal of Veterinary Diagnostic Investigation, 2011, 23(1):139-142.
[22] Zimmerman J J著.赵德明,张仲秋,周向梅等主译.猪病学[M]. 第10版. 北京:中国农业大学出版社,2014.
[23] Luigi P, Silvia B, Carola M, et al.Erysipelothrix rhusiopathiae bacteremia without endocarditis:Rapid identification from positive blood culture by MALDI-TOF mass spectrometry. A case spectrometry. A case report and literature review[J].Infectious Disease Reports, 2016, 8(1):6368.
[24] 谭侃侃.丹毒丝菌的分离鉴定及其SpaA-N蛋白和1iPo蛋白的免疫原性分析[D].长沙:湖南农业大学,2011.
[25] 张乐宜,蔡汝健,蒋智勇,等.猪红斑丹毒丝菌GZ株的分离鉴定及序列分析[J].西北农业学报,2014,23(7):31-38.

Isolation and Identification of Erysipelothrix rhusiopathiae from Abattoirs in Shanghai Area

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