沼泽型水牛微卫星DNA的遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2016.11.027

Abstract

Abstract:

This study was aimed to investigate the genetic diversity of swamp buffalo breeds in China.The analysis of genetic diversity was performed in 40 buffalo individuals from 8 buffalo breeds（Dechang buffalo,Dehong buffalo,Wenzhou buffalo,Guizhou buffalo,Xilin buffalo,Fuzhong buffalo,Murrah buffalo and Nili-Ravi buffalo）by 30 microsatellite loci and LabChip chip test method.The results showed that 332 alleles at 30 microsatellite loci were found in 8 buffalo breeds,the average values of gene diversity and PIC were 0.7808 and 0.7554,respectively.Cluster analysis indicated that Dechang buffalo and Dehong buffalo firstly clustered together,followed by Fuzhong buffalo,Guizhou buffalo,Wenzhou buffalo and Xilin buffalo.Moreover,Murrah buffalo and Nili-Ravi buffalo clustered together.Our findings revealed that the 30 microsatellite loci could be used as an effective genetic marker for the analysis of genetic diversity among the buffalo breeds,which enriched the current SSR marker resources in buffalo.

Key words: buffalo; microsatellite; genetic diversity; chip test

CLC Number:

S813.3

DENG Ting-xian, PANG Chun-ying, ZHU Peng, LU Xing-rong, DUAN An-qin, LIANG Xian-wei. Genetic Diversity Analysis of Microsatellite DNA Markers in Swamp Buffalo[J]. , 2016, 43(11): 2989-2996.

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Genetic Diversity Analysis of Microsatellite DNA Markers in Swamp Buffalo

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