›› 2016, Vol. 43 ›› Issue (8): 2176-2182.doi: 10.16431/j.cnki.1671-7236.2016.08.034

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Analysis of Ribosome Genotyping and Antimicrobial Resistance in Enterococcus faecalis Isolated from Pigs and Chickens

QIAN Xiao-lu, SHANG Jun, TIAN Kai, ZHANG Hao-ran   

  1. Shanghai Institute for Veterinary Drug & Feeds Control, Shanghai 201103, China
  • Received:2016-01-25 Online:2016-08-20 Published:2016-08-23

Abstract:

This study was to investigate the ribosome genotyping and antimicrobial resistance in Enterococcus faecalis strains isolated from 2 large-scale farms in Shanghai.40 isolates were evaluated for their sensitivity to 12 antimicrobial agents by broth microdilution,and the ribosome genotyping (ribotype) was characterized by the Riboprinter® Microbial Characterization System.The resistance rates of most antimicrobial drug were relatively high and multidrug resistant strains were detected with more than 80%.4 strains of Enterococcus faecalis isolated from pigs were resistant to vancomycin,including 2 strains of vancomycin highly resistant (MIC>64 mg/L) with the same time highly resistant to gentamicin and streptomycin (MIC>2 048 mg/L).The results showed that multidrug resistance of Enterococcus faecalis was a serious issue,and the resistant phenotypes of the same type of ribotype was not entirely consistent.

Key words: Enterococcus faecalis; antimicrobial resistance; ribosome genotyping; antimicrobial susceptibility test

CLC Number: