猪接触传染性胸膜肺炎放线杆菌血清3型分子鉴定及药敏试验

doi:10.16431/j.cnki.1671-7236.2016.01.041

Abstract

Abstract: In order to understand the serotype and drug resistance of porcine contagious Actinobacillus pleuropneumoniae(APP), a pair of primers was designed according to GenBank database to amplify specific 950 bp fragment, and the molecular identification and antimicrobial susceptibility test of APP serotype 3 were investigated.The results showed that the PCR product sequences were more than 99% homology with the APP serotype 3 published in GenBank.The isolated strains were highly resistant and multiple drug resistance.The molecular identification and antimicrobial susceptibility test of APP serotype 3 provided the basis for the identification, diagnosis and prevention of porcine contagious pleuropneumonia.

Key words: porcine contagious pleuropneumonia; Actinobacillus pleuropneumoniae; serotype 3; antimicrobial susceptibility test

CLC Number:

S852.61⁺9

LI Hai-li, XU Yin-di, SONG Yu-min, ZHU Wen-hao, ZHANG Qing-xian, WANG Ke-ling, FENG Ya-jie, HOU Zi-hua. Molecular Identification and Antimicrobial Susceptibility Test of Porcine Contagious Actinobacillus pleuropneumoniae Serotype 3[J]. , 2016, 43(1): 280-284.

References

[1] Yang X,Cheng Y T,Tan M F,et al.Overexpression of porcine Beta-defensin 2 enhances resistance to Actinobacillus pleuropneumoniae infection in pigs[J]. Infect Immun,2015,83(7):2836-2843.
[2] Yang F,Xie F,Jiang Y,et al.Genomic differences between Actinobacillus pleuropneumoniae serotypes 5b and 3 and their distribution and transcription among 15 serotypes[J]. Curr Microbiol,2011,63(4):327-331.
[3] Grasteau A,Tremblay Y D,Labrie J,et al.Novel genes associated with biofilm formation of Actinobacillus pleuropneumoniae[J]. Vet Microbiol,2011,153(1-2):134-143.
[4] Wang L,Qin W,Yang S,et al.The Adh adhesin domain is required for trimeric autotransporter Apa1-mediated Actinobacillus pleuropneumoniae adhesion,autoaggregation,biofilm formation and pathogenicity[J]. Vet Microbiol,2015,177(1-2):175-183.
[5] Wang L,Qin W,Ruidong Z,et al.Differential gene expression profiling of Actinobacillus pleuropneumoniae during induction of primary alveolar macrophage apoptosis in piglets[J]. Microb Pathog,2015,78:74-86.
[6] Pereira M F,Rossi C C,de Queiroz M V,et al. Galleria mellonella is an effective model to study Actinobacillus pleuropneumoniae infection[J]. Microbiology,2015,161(2):387-400.
[7] Pereira M F,Rossi C C,de Carvalho F M,et al.Draft genome sequences of six Actinobacillus pleuropneumoniae serotype 8 Brazilian clinical isolates:Insight into new applications[J]. Genome Announc,2015,3(2):e01585-14.
[8] Perry M B,Angen O,MacLean L L,et al.An atypical biotypeⅠ Actinobacillus pleuropneumoniae serotype 13 is present in North America[J]. Vet Microbiol, 2012,156(3-4):403-410.
[9] Archambault M,Harel J,Goure J,et al.Antimicrobial susceptibilities and resistance genes of Canadian isolates of Actinobacillus pleuropneumoniae[J]. Microb Drug Resist,2012,18(2):198-206.
[10] Kang S,Li Z,Yin Z,et al.The antibacterial mechanism of berberine against Actinobacillus pleuropneumoniae[J]. Nat Prod Res,2015,29(23):2203-2206.
[11] Ito H,Sueyoshi M.The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 15[J]. J Vet Med Sci, 2015,77(4):483-486.
[12] Ito H,Matsumoto A.Isolation and genetic characterization of an Actinobacillus pleuropneumoniae serovar K12:O3 strain[J]. J Vet Diagn Invest,2015,27(1):102-106.
[13] Vanni M,Merenda M,Barigazzi G,et al.Antimicrobial resistance of Actinobacillus pleuropneumoniae isolated from swine[J]. Vet Microbiol,2012,156(1-2):172-177.
[14] Tobias T J,Bouma A,Klinkenberg D,et al.Detection of Actinobacillus pleuropneumoniae in pigs by Real-time quantitative PCR for the apxIVA gene[J]. Vet J,2012,193(2):557-560.
[15] Ito H.The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 14[J]. J Vet Med Sci,2015,77(5):583-586.
[16] Hathroubi S,Fontaine-Gosselin S E,Tremblay Y D,et al.Sub-inhibitory concentrations of penicillin G induce biofilm formation by field isolates of Actinobacillus pleuropneumoniae[J]. Vet Microbiol,2015,179(3-4):277-286.
[17] Dayao D A,Dawson S,Kienzle M J,et al.Variation in the antimicrobial susceptibility of Actinobacillus pleuropneumoniae isolates in a pig,within a batch of pigs,and among batches of pigs from one farm[J]. Microb Drug Resist, 2015,21(4):491-496.
[18] Chang N Y,Chen Z W,Chen T H,et al.Elucidating the role of ApxI in hemolysis and cellular damage by using a novel apxIA mutant of Actinobacillus pleuropneumoniae serotype 10[J]. J Vet Sci,2014,15(1):81-89.
[19] Bosse J T,Li Y,Angen O,et al.Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3,5 to 8,10,and 12[J]. J Clin Microbiol,2014,52(7):2380-2385.
[20] Yoo A N,Cha S B,Shin M K,et al.Serotypes and antimicrobial resistance patterns of the recent Korean Actinobacillus pleuropneumoniae isolates[J]. Vet Rec,2014,174(9):223.
[21] Turni C,Singh R,Schembri M A,et al.Evaluation of a multiplex PCR to identify and serotype Actinobacillus pleuropneumoniae serovars 1,5,7,12 and 15[J]. Lett Appl Microbiol, 2014,59(4):362-369.
[22] Hur J,Lee J H.Optimization of immune strategy for a construct of Salmonella-delivered ApxIA,ApxIIA,ApxⅢA and OmpA antigens of Actinobacillus pleuropneumoniae for prevention of porcine pleuropneumonia using a murine model[J]. Vet Res Commun,2014,38(1):87-91.
[23] Gottschalk M,Lacouture S. Actinobacillus pleuropneumoniae serotypes 3,6,8 and 15 isolated from diseased pigs in North America[J]. Vet Rec, 2014,174(18):452.
[24] Marois-Crehan C,Lacouture S,Jacques M,et al.Development of two Real-time polymerase chain reaction assays to detect Actinobacillus pleuropneumoniae serovars 1-9-11 and serovar 2[J]. J Vet Diagn Invest, 2014,26(1):146-149.
[25] Ito H,Katsuragi K,Akama S,et al.Isolation of atypical genotype Actinobacillus pleuropneumoniae serotype 6 in Japan[J]. J Vet Med Sci, 2014,76(4):601-604.
[26] Klinkenberg D,Tobias T J,Bouma A,et al.Simulation study of the mechanisms underlying outbreaks of clinical disease caused by Actinobacillus pleuropneumoniae in finishing pigs[J]. Vet J,2014,202(1):99-105.

Molecular Identification and Antimicrobial Susceptibility Test of Porcine Contagious Actinobacillus pleuropneumoniae Serotype 3

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