华中地区鸡源沙门菌分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2019.03.035

Abstract

Abstract:

In order to explore the dominant serovars and antimicrobial resistance of avian Salmonella spp.in Central China,a total of 3 724 issue samples were collected from diseased chickens,dead embryos or weak chick in 22 large-scale farms in Hubei,Henan and Hunan,etc.The isolates and their serotypes were identified by PCR and serotype assay.Kirby-Bauer method was used to analyze the drug resistance of the isolates.The results showed that 124 Salmonella were identified from 3 724 samples,among those 79 strains were Salmonella Enteritidis of D serogroup (63.71%,79/124),34 strains were Salmonella Pullorum of D serogroup (27.42%,34/124),8 strains were Salmonella Typhimurium of B serogroup (6.45%,8/124),whereas 3 strains were failed to be determined.The O antigen of 79 Salmonella Enteritidis and 34 Salmonella Pullorum strains were identified O9 antigen,that of 8 Salmonella Typhimurium strains were O4 antigen.While the H antigen of Salmonella Enteritidis and Salmonella Pullorum strains were identified Hg,m,that of Salmonella Typhimurium strains were Hi.The results of antimicrobial susceptibility test showed that the resistance rate of isolates to nalidixic acid,ampicillin,tetracycline and doxycycline were 95.97% (119/124),91.94% (114/124),57.26% (71/124) and 70.16% (87/124),respectively.The resistance rate to Timethoprim/sulfamethoxazole and erythromycin were 25.81% (32/124) and 12.10% (15/124).The resistance rate to chloramphenicol,gentamicin,cefotaxime and kanamycin were 6.45% (8/124),1.61% (2/124),1.61% (2/124) and 0.81% (1/124),respectively,whereas all isolates were completely sensitive to levofloxacin,amikacin and polymyxin B.Altogether,99.19% (123/124) of these isolates were resistant to at least one drug,and 87.10% (108/124) of isolates showed multiple drug resistance.This study provided the basic data for the diagnosis and prevention of Salmonella in poultry farms in Central China.

Key words: Salmonella; isolation and identification; serovar; antimicrobial resistance

CLC Number:

S852.6

WANG Dixuan, GAO Dongyang, ZHANG Tianzi, LIU Wei, ZHOU Zutao, XU Xiaojuan, CAI Xuwang. Isolation,Identification and Drug Resistance Analysis of Avian Salmonella in Central China[J]. , 2019, 46(3): 931-939.

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Isolation,Identification and Drug Resistance Analysis of Avian Salmonella in Central China

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