›› 2019, Vol. 46 ›› Issue (4): 1199-1208.doi: 10.16431/j.cnki.1671-7236.2019.04.029

• Basic Veterinary Medicine • Previous Articles     Next Articles

Analysis of Epidemicity and Antimicrobial Resistance of Staphylococcus aureus Isolated from Mastitis Milk in Xinjiang and Inner Mongolia

YU Zhongna1, JIA Lingyun2, ZHEN Tianyuan2, WANG Jun2, HAN Rongwei2   

  1. 1. Haidu College, Qingdao Agricultural University, Laiyang 265200, China;
    2. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2018-09-18 Online:2019-04-20 Published:2019-04-22

Abstract:

To evaluate the epidemicity and antimicrobial resistance of Staphylococcus aureus in the milk of mastitis cows,70 samples from Xinjiang and 50 samples from Inner Mongolia were collected from April to May in 2018.Staphylococcus aureus was cultivated,separated and purified.Antibiotic susceptibility test of purified isolates with KB paper and PCR analysis for the drug resistance gene were carried out.The results showed that the separation rate of Staphylococcus aureus in Xinjiang and Inner Mongolia was 28.6% and 54.0%,respectively.Penicillin G (75.0%) was the highest resistant antibiotic for Staphylococcus aureus isolated from Xinjiang,followed by sulfis oxazole (60.0%),lincomycin (55.0%).For the isolates from Inner Mongolia,penicillin G (70.4%) was also the highest resistant drug,followed by lincomycin (63.0%) and clindamycin (51.9%).The results of the resistance gene test showed that multi-resistance genes were detected in more than 88.9% of the isolates.The highest frequency resistance genes of isolated were ermA (30.0%) and dfrS1(30.0%) in Xinjiang and lnuA (46.2%) in Inner Mongolia,respectively.

Key words: mastitis; Staphylococcus aureus; raw milk; epidemicity; antimicrobial resistance

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