基于网络药理学方法研究阿司匹林丁香酚酯防治犬心血管疾病的作用机制

doi:10.16431/j.cnki.1671-7236.2019.04.028

Abstract

Abstract:

The target,gene enrichment and pathway of aspirin and eugenol were investigated by network pharmacological method in order to speculate the action mechanism of aspirin eugenol ester (AEE) on preventing and curing canine cardiovascular diseases.From methodology,aspirin and eugenol were searched from DrugBank database to obtain their target,and then the compound-target network,the canine protein-protein interaction (PPI) network and target-pathway network were constructed.Then the targets were uploaded to the STRING database,the species was selected as canine,and the PPI network diagram was obtained.Finally,the targets acting on canine were uploaded to the DAVID database,and the species was selected as canine.Gene enrichment and KEGG pathway with false discovery rate <0.01 were screened out.These networks were used to speculate the mechanism of action of AEE on preventing and curing canine cardiovascular diseases.The results showed that there were 15 known targets related to aspirin and eugenol,of which 8 were corresponding with canine.Key targets included TP53 gene,NF-κB,androgen receptor and prostaglandin G/H synthetase 1.There were 4 gene function enrichment (GO) entries,including 2 molecular functions,involving steroid binding and transcription factor activity,sequence specific DNA binding,and 2 biological processes,involving DNA transcription template and its positive regulation.There were 2 KEGG pathways involved in prostate cancer and cancer signaling pathways.This study suggested that AEE might regulate TP53 gene,NF-κB,androgen receptor,prostaglandin G/H synthetase 1,etc.Gene functions were enriched in DNA template transcription,steroid binding,transcription factor activity and specific DNA sequence binding,and positive regulation of DNA template transcription.AEE could use prostate cancer and other cancer signaling pathways to treat canine cardiovascular disease.

Key words: aspirin eugenol ester (AEE); aspirin; eugenol; network pharmacology

CLC Number:

R285.5

JIAO Yuting, JIAO Zenghua, YANG Xiaopu, LI Jianyong. Mechanism of AEE in Preventing and Curing Canine Cardiovascular Diseases Based on Network Pharmacological Method[J]. , 2019, 46(4): 1191-1198.

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Mechanism of AEE in Preventing and Curing Canine Cardiovascular Diseases Based on Network Pharmacological Method

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