貉源肺炎克雷伯菌的分离鉴定及药敏试验

doi:10.16431/j.cnki.1671-7236.2019.04.030

Abstract

Abstract:

To determine the pathogen caused a serious mortality of racoon dog in Hebei province,and one Gram-negative bacterium was isolated from the the lung samples of diseased racoon dog.The isolate received morphological obsrvation,16S rRNA sequence analysis and biochemical test.Basis on this,the serotype characteristics,pathogenicity tests,drug sensitivity test were completed.The isolated strain was confirmed as not K1/K2 Klebsiella pneumoniae,named as CLKp18.16S rRNA gene sequence analysis results showed that the bacterium was 99% homologous with many strains of Klebsiella accessed in GenBank.The Vitek automatic bacterial identification results showed that the similarity between the strain and Klebsiella pneumoniae standard was 99%.Pathogenicity test results showed that the mice in the experimental group began to develop clinical symptoms 6 h after the challenge.The lung,liver,spleen and kidney of the dead mice were aseptically removed,and the bacteria were isolated by scribing and plating.It indicated that the infected strain could invade many tissues and organs in mice after infection,which caused different degrees of lesions,especially liver and lung lesions,the LD₅₀ in mice was 2.17×10⁷ CFU.Drug susceptibility test results showed that the strain resistant to monocyclic lactam,chloramphenicol,aminoglycosides,tetracyclines,sulfonamides and most cephalosporins,and sensitive to amikacin,amoxycillin/clavulanic acid,imipenem and ceftazidime.This study confirmed that K1/K2 Klebsiella pneumoniae didn't cause the pathogens,and the strain with strong pathogenicity in mice and multi-drug resistance.The results provided a reference for clinical treatment of pneumonia caused by the strain.

Key words: racoon dog; Klebsiella pneumonia; isolation and identification; drug susceptibility test

CLC Number:

S852.61⁺2

HAN Kun, LYU Jiao, YU Ying, WANG Yang, WANG Yawen, XU Hao, CHEN Lizhi, FENG Erkai, BAI Xue, YAN Xijun. Isolation,Identification and Drug Susceptibility of Klebsiella pneumoniae Isolated from Racoon Dog[J]. , 2019, 46(4): 1209-1216.

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Isolation,Identification and Drug Susceptibility of Klebsiella pneumoniae Isolated from Racoon Dog

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