葫芦多糖体外抗鸡新城疫病毒作用的研究

doi:10.16431/j.cnki.1671-7236.2016.07.032

Abstract

Abstract:

To research on polysaccharides from Lagenaria siceraria (Molina) Standl.against Newcastle disease virus (NDV).Total polysaccharide and four different solvent extractions of Lagenaria siceraria (Molina) Standl.were extracted by the methods of hot water extraction and ethanol precipition in this study.The content of polysaccharide was measured by phenol-sulfuric acid method and infrared spectroscopy method.The safe concentration and growth of chick embryo fibroblast (CEF) were assayed by MTT method,in order to facilitated the comparison under the same level,the safe concentration was united as 78.125 μg/mL.Under the safety range of concentration,detected the block-virus activity,anti-virus activity and virus-killing activity of polysaccharides through the ways of pre-adding polysaccharides,post-adding polysaccharides and adding polysaccharides with NDV.The results showed that the direct inactivation and propagation inhibition activity of total polysaccharide and four different solvent extractions were stronger than anti-absorption function.Anti-virus inhibition rate of 70%,80% gradient alcohol precipitation of polysaccharides from Lagenaria siceraria (Molina) Standl.(LSP₇₀,LSP₈₀) and total polysaccharide (LSP_t)were 40.41%,44.54% and 61.85%,virus-killing inhibition rate were 44.74%,58.76% and 59.38%.LSP₈₀ had the highest virus inhibition rate as 37.14% in the block-virus activity of those five polysaccharides.In summary,70%,80% gradient alcohol precipitation and total polysaccharide in polysaccharides possessed better activity and would be as the materials for further research.

Key words: Lagenaria siceraria (Molina) Standl.; polysaccharide; chick embryo fibroblast; Newcastle disease virus

CLC Number:

S853.73

ADELIJIANG Wusiman, DELIBAIER, NUERAILI Maitiniyazi, BUHELIQIANMU Yiming, SAIFUDING Abula. Study on Antiviral Effect of Polysaccharides from Lagenaria siceraria (Molina) Standl.on NDV in vitro[J]. , 2016, 43(7): 1869-1875.

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Study on Antiviral Effect of Polysaccharides from Lagenaria siceraria (Molina) Standl.on NDV in vitro

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