香猪SNP位点rs80894395的多态性及其与体尺指标之间的关联研究

doi:10.16431/j.cnki.1671-7236.2016.05.026

Abstract

Abstract: To explore the functional genes affecting the growth and development of Xiang pig, a single nucleotide polymorphism (SNP) site rs80894395, located at intron 10 of polypeptide N-acetylgalactosaminyltransferase 2 (GALNT2) gene was screened out by Illumina Porcine SNP60K chip, which showed a weak association with the abdomen circumference of pig.Further, the genotypes of site rs80894395 in 520 female Xiang pigs were detected using allele-specific PCR(AS-PCR) method, taking 43 Yorkshire pigs and 36 Kele pigs as control groups.All of CC, CT and TT genotypes were detected from the three pig breeds.The dominant genotype of Xiang pig was CT genotype and it was positively weak-associated with its body measurement indexes including body weight, body length, and abdomen circumference.The CC genotype was dominant genotype in Yorkshire pig, and the frequency of T allele was extremely significant decreased compared with that of Xiang pig (P<0.01).There was no difference in the frequencies of CC and CT genotypes in Kele pig, and its allele frequencies was not significantly different compared with that of Xiang or Yorkshire pig breeds (P>0.05).A splice-site nearby site rs80894395 was found out using bioinformatics prediction software, which might affect the splicing process of GALNT2 gene.It suggested that site rs80894395 might have a connection with the growth and development of Xiang pig.

Key words: Xiang pig; body sizes; intron splicing; SNP; GALNT2 gene

CLC Number:

S828

WANG Yang, MEI Zi-ling, YU Chang-yan, NIU Xi, XIE Ling-ling, NIU Qiu-jin, QIU Zhi-lang, WANG Jia-fu, RAN Xue-qin. Polymorphism of SNP Site rs80894395 and its Association with the Body Size of Xiang Pig[J]. , 2016, 43(5): 1294-1301.

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Polymorphism of SNP Site rs80894395 and its Association with the Body Size of Xiang Pig

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