TLRs介导的信号通路在马链球菌感染RAW264.7细胞中的作用

doi:10.16431/j.cnki.1671-7236.2016.05.023

Abstract

Abstract: To investigate the effects of Toll-like receptors (TLRs) mediated signaling pathway in mouse macrophage RAW264.7 cells on Streptococcus equi subspecies equi (S.equi) infection, RAW264.7 cells were infected with S.equi for different time intervals.The mRNA expression levels of TLR1, TLR2, TLR6, myeloid differentiation factor 88 (MyD88), IL-1, IL-6, IL-10, IL-12 and TNF-α were detected by fluorescence quantitative Real-time PCR.The results showed that at 6 h post-infection, the expression levels of TLR1, TLR2, TLR6 and MyD88 in S.equi infected RAW264.7 cells had no significant difference compared with control group (P>0.05).At 12 h post-infection, the mRNA expression levels of TLR1, TLR2 and TLR6 had no obviously change(P>0.05), while MyD88 mRNA expression level was extremely significantly increased (P<0.01).At 24 h post-infection, the mRNA levels of TLR1, TLR2, TLR6 were extremely significantly increased (P<0.01), the mRNA level of MyD88 had no significant difference (P>0.05), meanwhile, compared with uninfected cells, IL-10 and IL-12 mRNA expression were extremely significant increased in S.equi infected RAW264.7 cells (P<0.01), but IL-1, IL-6 and TNF-α mRNA levels were extremely significantly decreased (P<0.01).These results indicated that the signaling pathway mediated by TLRs participated in the immune response against S.equi.

Key words: Streptococcus equi subspecies equi; RAW264.7 cells; Toll-like receptor; MyD88; cytokine

CLC Number:

Q2-33

DU Run-ci, LIU Jian-hua, SHI Qian, FU Qiang, RAN Duo-liang. Effect of TLRs Mediated Signal Pathway on Streptococcus equi Infection in RAW264.7 Cells[J]. , 2016, 43(5): 1270-1276.

References

[1] 郭纪珂,王晓钧.马腺疫[J].畜牧兽医科技信息,2013,10:16-18.
[2] Flock M,Jacobsson K,Frykberg L,et al.Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses[J].Infect Immun,2004,72(6):3228-3236.
[3] Flock M,Karlstrom A,Lannerqard J,et al.Protective effect of vaccination with recombinant proteins from Streptococcus equi subspecies equi in a strangles model in the mouse[J].Vaccine,2006,24(19):4144-4151.
[4] 徐全圆.马腺疫链球菌的分离鉴定及FN结合蛋白免疫原性的研究[D].乌鲁木齐:新疆农业大学,2014.
[5] 马思慧,杨欢,吴天成,等.Toll样受体信号传导通路的研究进展[J].中国畜牧兽医,2014,8(41):160-164.
[6] 郎需龙,冉会玲,王秀然,等.猪链球菌2型分解代谢控制蛋白A经NF-κB/MAPK通路对巨噬细胞RAW264.7影响的研究[J].中国畜牧兽医,2013,7(40):21-24.
[7] Miller L S.Toll-like receptors in skin[J].Adv Dermatol,2008,24(24):71-87.
[8] Takeda K,Kaisho T.Toll-like receptors[J].Annu Rev Immunol,2003,21(1):335-376.
[9] Takeuchi O,Hoshino K,Kawai T,et al.Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive cell wall components[J].Immunity,1999,11(4):443-451.
[10] Echchannaoui H,Frei K,Schnell C,et al.Toll-like receptor 2-deficient mice are highly susceptible to Streptococcus pneumoniae meningitis because of reduced bacterial clearing and enhanced inflammation[J].J Infect Dis,2002,186(6):798-806.
[11] Takeuchi O,Hoshino K,Akira S.Cutting edge:TLR2-deficient and MyD88 deficient mice are highly susceptible to Staphylococcus aureus infection[J].J Immunity,2000,165(10):5392-5396.
[12] Florindo H F,Pandit S,Goncalves L M D,et al.Streptococcus equi antigens adsorbed onto surface modified poly-ε-caprolactone microspheres induce humoral and cellular specific immune responses[J].Vaccine,2008,26(33):4168-4177.
[13] Parkinson N J,Robin C,Newton J R,et al.Molecular epidemiology of strangles outbreaks in the UK during 2010[J].Vet Rec,2011,168(25):666.
[14] Triantafilou M,Gamper F G.Membrane sorting of toll-like receptor(TLR)-2/6 and TLR2/1 heterodimers at the cell surface determines heterotypic associations with CD36 and intracellular targeting[J].Biol Chem,2006,281(41):31002-31011.
[15] Alexopoulou L,Holt A C,Medzhitov R,et al.Recognition of double-stranded RNA and activation of NF-kappa B by Toll-like receptor 3[J].Nature,2001,413(6875):732-738.
[16] Palsson-McDermott E M,O'Neill L A.Signal transduction by the lipoplysaccharide receptor,Toll-like receptor-4[J].Immunology,2004,62(2):113-153.
[17] Hayashi F,Smith K D,Ozinsky A,et al.The innate immune response to bacterial flagellin is mediated by toll-like receptor 5[J].Nature,2001,410(6832):1099-1103.
[18] Sandor F,Buc M.Toll-like receptors Ⅱ distribution and pathways involved in TLR signaling[J].Folia Biol(Praha),2005,51(6):188-197.
[19] Barton G M,Medzhitov R.Toll-like receptor signaling pathways[J].Science,2003,300(5625):1524-1525.
[20] Lecours M P, Segura M, Claude L, et al. Characterization of porcine dendritic cell response to Streptococcus suis[J].Veterinary Research,2011,42(42):1-12.
[21] Takeuchi O,Kaufmann A,Grote K,et al.Cutting edge:Preferentially the R-stereoisomer of the mycoplasmal lipopeptide macrophage-activating lipopeptide-2 activates immune cells through a Toll-like receptor 2 and MyD88 dependent signaling pathway[J].J Immunol,2000,164(2):554-557.
[22] Takeuchi O,Kawai T,Muhlradt P F,et al.Discrimination of bacterial lipoproteins by Toll-like receptor 6[J].Int Immunol,2001,13(7):933.
[23] Rönnberg E,Guss B,Pejler G.Infection of mast cells with live streptococci causes a Toll-like receptor 2 and cell-cell contact-dependent cytokine and chemokine response[J].Infect Immun,2010,78(2):854-864.
[24] 张晓燕,蒋小武,杨岭.HEp-2细胞感染猪链球菌2型后细胞因子mRNA表达变化[J].中国预防兽医学报,2014,36(9):696-700.

Effect of TLRs Mediated Signal Pathway on Streptococcus equi Infection in RAW264.7 Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[2]	FU Jingcheng, HE Junhui, TONG Jiang, WU Wei, GUO Shuang, YANG Yanbin, HAN Yingqian, WANG Yueying, LI Heping. Anti-inflammatory and Antioxidant Roles of HO-1 in Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 807-816.
[3]	XIE Jingjing, REN Mengting, FAN Jiahui, JIAO Yaru, HAN Zongxi, MA Deying. Study on Immune Response of Infectious Bronchitis Virus to Chickens and Antiviral Effect of Recombinant Avian Beta-defensins 11 and 12 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 246-259.
[4]	LIU Haoyuan, LI Yanjie, QIN Hewei, JIN Xiaoqin, NIU Yuqing, HUA Xiaoqiong, ZHANG Shuqin, NIU Li. Study on the Mechanism of WU Shen Venation Smooth Decoction on Atherosclerosis AopE^-/- Mice Based on Network Pharmacology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 377-389.
[5]	CHEN Shiyu, CUI Shuai, JIANG Yajun, XIN Ting, WANG Yang, HAO Yuxin, HUANG Jianxin, GUO Xiaoyu, ZHU Hongfei, WU Jiajun, JIA Hong. lncRNA-mRNA Analysis of Toll-like Receptor Signaling in Porcine Peripheral Blood Lymphocytes After African Swine Fever Virus Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2677-2687.
[6]	LIU Yuran, YANG Huiying, ZHU Yan, WANG Mengmeng, SUN Guojie, ZHANG Mengyuan, WANG Yan, GAO Jiahao, LIAN Kexun, GU Xinli. Study on the Anti-inflammatory Effect of ‘Ruyan Ning’ Water Extract [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1879-1887.
[7]	CHEN Yu, CHEN Xiaolan, YANG Haifeng, LUO Jun, ZHANG Ting, ZUO Haiping, ZHENG Yi. Therapeutic Effect of Wuwei Zhili Mixture on Diarrhea Induced by Escherichia coli in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 2001-2009.
[8]	QIU Shulei, CHEN Xiaolan, YANG Haifeng, CHEN Yuku, HU Yuanliang, GAO Yongxu, WU Caihong. Effects of Selenized Garlic Polysaccharide on the Function of Mouse Peritoneal Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1144-1152.
[9]	YIN Huihui, TONG Yanmei, ZENG Xueyan, LU Guangqiang, ZHAO Wu, JIANG Yuanming, LIU Wei. Effect of Polysaccharide from Rhodomyrtus tomentosa Fruit on Immunological Function in Immunosuppressed Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 731-737.
[10]	ZHAO Yi, ZHANG Wen, CHEN Jiaji, SONG Manling, CHEN Qi, XIE Xiaodong, HU Tingjun. Establishment of 3D4/2 Cell Inflammatory Model Induced by PCV2 in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4920-4927.
[11]	ZHU Yixuan, LIU Shipeng, ZHANG Wei, LI Jinrong, PENG Fei, LIN Na, WANG Yuyan, LIU Dandan. Study on the Optimization of Modified Formula for Inhibiting Streptococcus equi subspecies equi and Its Bacteriostatic Effect in Vivo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4085-4096.
[12]	CHANG Hua, DUAN Gang, RUAN Qian, LUO Qianmin, YU Wanling, LIU Qingqi, HUANG Cuiqin, XIANG Xun. Cloning and Bioinformatics Analysis of TLR7 Gene in Larus ridibundus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 43-52.
[13]	YAN Ping, LIN Shaoqing, LI Minghui, SUN Xuening, LI Jian, HUANG Yifan, WU Yijian. Effects of Hericium erinaceus Polysaccharide on TLR3/TRIF-induced Expression and Viral Replication in MDRV-infected RAW264.7 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3415-3422.
[14]	ZHANG Jing, QIAN Yinghong, ZHANG Kai, WU Jindi, LIU Bo, MAO Wei, CAO Jinshan. Effects of Staphylococcus aureus Lipoprotein on Immunity in M1 Mouse Bone Marrow-Derived Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3079-3086.
[15]	QIAO Lianjiang, CHEN Chen, ZHANG Ru, CUI Liheng, XU Li, YANG Yanling. Construction of Eukaryotic Expression Vectors and Bioinformatics Analysis of Rhoa and Ptgs2 Genes in RAW264.7 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2313-2323.