牛泰勒虫的分离鉴定及进化分析

doi:10.16431/j.cnki.1671-7236.2016.04.030

Abstract

Abstract: Bovine theileriosis was a kind of hemo-protozoan diseases that seriously hindered the sustainable development of cattle industry.In the present study, the infection of Theileria was detected using microscopic examination and species-specifc PCR of T.annulata, T.sergenti and T.sinensis from 18 blood samples collected from a breeding cattle farm in China.Then ITS and MPSP genes were amplified and cloned using universal primers of ITS and allele-specific primers of 4 major piroplasm surface protein (MPSP) from the positive samples.The sequences were used to make alignment, polymorphism and phylogeny analysis.The results showed that 3 samples were positive for Theileria and the 3 positive samples were all the T.sergenti infection.The amplification of allelic MPSP gene of T.sergenti from the 3 positive samples showed that two of them were single infection by Ikeda-type and another one was co-infection with both Chitose-type and Ikeda-type, while Buffeli-type and Thai-type were not detected.Moreover, on the basis of phylogenetic tree constructed with MPSP gene sequences, types 1 and 2 of MPSP were confirmed to be present in the cattle farm.The results revealed that there were T.sergenti infection in the breeding cattle farm, and these parasites at least with 2 allelic MPSP gene types were present, which indicated that the immune prevention and control of the disease became more complicated.Our research laid foundation of the further study on T.sergenti infection and disease prevention and control.

Key words: bovine theileriosis; microscopic examination; PCR; ITS gene; allelic MPSP gene

CLC Number:

S852.71

LIU Juan, LIU Jun-long, LIU Ai-hong, ZHAO Hong-xi, YANG Cong-shan, ZHAO Shuai-yang, LIU Guang-yuan, YIN Hong, GUAN Gui-quan, LUO Jian-xun. The Isolation,Identification and Phylogenetic Analysis of Theileria[J]. , 2016, 43(4): 1050-1057.

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The Isolation,Identification and Phylogenetic Analysis of Theileria

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