白洗猪PID1基因克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2016.04.008

Abstract

Abstract: In order to study phosphotyrosine interaction domain containing (PID1) gene of Baixi pig, it was amplified and sequenced by Nest PCR and T clone technology.Then the functions and the genetic evolutionary relationships of the gene and its predicted protein were analyzed by bioinformatics software.The results showed that the whole CDS region of PID1 gene was 654 bp, which encoded 217 amino acids.The results of sequence alignments showed that Baixi pig shared 98.2% and 97.7% similarities of amino acid with which of Shandong Laiwu pig and Guangxi Luchuan pig.The phylogenetic tree indicated that Baixi pig kept away from these two pigs.The results of sequence alignments among species showed that Baixi pig shared 96.6%, 96.6%, 96.3%, 95.0%, 93.9%, 91.7%, 90.8%, 88.2%, 69.7% and 67.3% similarities of amino acid with which of Bos taurus, Bos grunniens, Macaca mulatta, Mus musculus, Ophiophagus hannah, Homo sapiens, Gallus gallus, Xenopus laevis, Rattus norvegicus and Danio rerio.Phylogenetic analysis indicated that PID1 gene was highly conserved in the process of evolution of different species.The protein structure analysis results showed that the mainly function region of PID1 gene was PTB structural domain, which was located in the C-terminal sequence of the PID1 protein.In this study, we successfully cloned PID1 gene of Baixi pig, which laid the foundation for further study in intramuscular fat deposition and development of resources.

Key words: Baixi pig; meat quality; intramuscular fat; PID1 gene; clone; PTB

CLC Number:

S828

FENG Wen-wu, SUN Zhen-mei, LI Peng-cheng, DING Mei, XU Hou-qiang, ZHAO Jia-fu, CHEN Xiang. Cloning and Sequence Analysis of PID1 Gene in Baixi Pig[J]. , 2016, 43(4): 906-912.

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Cloning and Sequence Analysis of PID1 Gene in Baixi Pig

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