基于芯片数据和整合分析研究奶牛乳房炎的关键通路

doi:10.16431/j.cnki.1671-7236.2016.04.003

Abstract

Abstract: Dairy cow mastitis was one of the common diseases and frequently-occurring diseases, caused huge economic losses, and also severely restricted the development of dairy industry.To further understand the regulation mechanism of mastitis, published five sets of related microarray data sets of cow mammary epithelial cells infected by Escherichia coli were collected, preprocessed with the standardized method, and integrat the result of gene set enrichment analysis with the infection time of 1, 6 and 24 h, so as to get more reliable key genes and pathways which could affect dairy cow mastitis.We find 9 down-regulated pathways common in the infected 1 h data sets, 30 down-regulated pathways common in the infected 6 h data sets, 17 down-regulated pathways common in the infected 24 h data sets.After analysis, we identified a number of dairy cow mastitis related pathways and genes, which would provide a reference for further study of regulation mechanisms.

Key words: dairy cow; mastitis; gene set enrichment analysis; pathway

CLC Number:

S857.2⁺6

ZHAO Jing, CHEN Zhen-liang, WANG Zhen, ZHANG Zhe, XIAO Qian, GUAN Yu-yu, SUN Hao, LIU Guang-lei, ZHANG Xiang-zhe, PAN Yu-chun, LIAO Rong-rong, WANG Qi-shan. Study on the Critical Pathways of Dairy Cow Mastitis Based on Microarray Dataset and Integration Analysis[J]. , 2016, 43(4): 862-869.

References

[1] Mootha V K,Lindgren C M,Eriksson K F,et al.PGC-1α-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes[J].Nature Genetics,2003,4(3):267-273.
[2] Brand B,Hartmann A,Repsilber D,et al.Comparative expression profiling of E.coli and S.aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score[J].Genet Sel Evol,2011,43(1):24.
[3] Buitenhuis B,Røntved C M,Edwards S M,et al.In depth analysis of genes and pathways of the mammary gland involved in the pathogenesis of bovine Escherichia coli-mastitis[J].BMC Genomics,2011,12(1):130.
[4] Günther J,Petzl W,Zerbe H,et al.Lipopolysaccharide priming enhances expression of effectors of immune defence while decreasing expression of pro-inflammatory cytokines in mammary epithelia cells from cows[J].BMC Genomics,2012,13:17.
[5] Günther J,Esch K,Poschadel N,et al.Comparative kinetics of Escherichia coli-and Staphylococcus aureus-specific activation of key immune pathways in mammary epithelial cells demonstrates that S.aureus elicits a delayed response dominated by interleukin-6(IL-6) but not by IL-1A or tumor necrosis factor alpha[J].Infection and Immunity,2011,79(2):695-707.
[6] Mitterhuemer S,Petzl W,Krebs S,et al. Escherichia coli infection induces distinct local and systemic transcriptome responses in the mammary gland[J].BMC Genomics,2010,11(1):138.
[7] R Development Core Team.R:A language and environment for statistical computing[M].R Foundation for Statistical Computing,Vienna,Austria,2011.
[8] Gentleman R C,Carey V J,Bates D M,et al.Bioconductor:Open software development for computational biology and bioinformatics[J].Genome Biology,2004,5(10):R80.
[9] Gautier L,Cope L,Bolstad B M,et al.Affy-analysis of Affymetrix GeneChip data at the probe level[J].Bioinformatics,2004,20(3):307-315.
[10] Irizarry R A,Hobbs B,Collin F,et al.Exploration,normalization,and summaries of high density oligonucleotide array probe level data[J].Biostatistics,2003,4(2):249-264.
[11] Zhao H,Wang Q,Bai C,et al.A cross-study gene set enrichment analysis identifies critical pathways in endometriosis[J].Reprod Biol Endocrinol,2009,7:94.
[12] 赵洪波.子宫内膜相关芯片的通路富集分析及其在猪繁殖机理研究中的应用[D].上海:上海交通大学,2010.
[13] 牛丽莉.奶牛乳房炎相关血浆蛋白质初步分析[J].北京:中国农业科学院,2009.
[14] Kawai T,Akira S.Toll-like receptor and RIG-1-like receptor signaling[J].Annals of the New York Academy of Sciences,2008,1143(1):1-20.
[15] Bomford R,Henderson B.Interleukin-1,Inflammation and Disease[M].Elsevier:Sole distributors for the USA and Canada,Elsevier Science Pub.Co.,1989.
[16] Trigo G,Ferreira P,Ribeiro N,et al.Identification of immunoreactive extracellular proteins of Streptococcus agalactiae in bovine mastitis[J].Canadian Journal of Microbiology,2008,54(11):899-905.
[17] Lund F E.Cytokine-producing B lymphocytes-key regulators of immunity[J].Current Opinion in Immunology,2008,20(3):332-338.
[18] De Schepper S,De Ketelaere A,Bannerman D D,et al.The Toll-like receptor-4(TLR-4) pathway and its possible role in the pathogenesis of Escherichia coli mastitis in dairy cattle[J].Veterinary Research,2008,39(1):1-23.
[19] Akira S,Uematsu S,Takeuchi O.Pathogen recognition and innate immunity[J].Cell,2006,124(4):783-801.
[20] 罗兵,李涛,徐元宏.Toll样受体的信号转导及抗感染免疫研究进展[J].免疫学杂志,2011,27(2):165-169.
[21] Cascieri M A,Springer M S.The chemokine/chemokine-receptor family:Potential and progress for therapeutic intervention[J].Current Opinion in Chemical Biology,2000,4(4):420-427.
[22] Yoshie O,Imai T,Nomiyama H.Chemokines in immunity[J].Advances in Immunology,2001,78:57-110.
[23] Usman T.JAK-STAT通路基因对奶牛乳房炎抗性的遗传和表观遗传效应研究[D].北京:中国农业大学,2014.
[24] 韩艳春,曾宪旭,王睿,等.p38MAPK信号通路与uPA在乳腺癌细胞及组织中表达的相关性[J].癌症,2007,26(1):48-53.
[25] Tomaszewski J E,Brooks J S,Hicks D,et al.Diabetic mastopathy:Adistinctive clinicopathologic entity[J].Human Pathology,1992,23(7):780-786.
[26] 吴金梅.甘草总黄酮抗金黄色葡萄球菌作用及其治疗奶牛乳房炎的应用研究[J].长春:吉林大学,2012.

Study on the Critical Pathways of Dairy Cow Mastitis Based on Microarray Dataset and Integration Analysis

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