莱菔硫烷对镉中毒雄性小鼠生殖机能保护作用的研究

doi:10.16431/j.cnki.1671-7236.2016.02.027

Abstract

Abstract: The study was aimed to explore the protective effect of sulforaphane (SFN) on the reproductive function of male mice with cadmium poisoning.40 healthy clean grade male Kunming mice were randomly divided into four groups:control group (H₂O),cadmium chloride group (2.3 mg/kg CdCl₂),sulforaphane group (10 mg/kg SFN),sulforaphane + cadmium chloride group (10 mg/kg SFN+2.3 mg/kg CdCl₂),and continuous administration for 10 d,all mice were executed by dislocated cervical vertebra at 2 d after the last administration,and then the pathologic changes of testicular tissues,organ coefficient of testicle and epididymis,sperm quality and concentration of testosterone were tested.Additionally,the contents of GSH and MDA,and the activities of T-SOD in testis were also detected at the same time. Compared with the control group,pathology damages were observed in cadmium chloride group,organ coefficient of testis and epididymis,sperm quality and levels of testosterone extremely significantly decreased (P<0.01),the activities of T-SOD and GSH content were extremely significantly decreased (P<0.01),and the concentration of MDA was extremely significantly enhanced (P<0.01).Compared with the control group,the activity of T-SOD and concentration of GSH in sulforaphane group were significantly increased (P<0.05),and the concentration of MDA was not significant different between the control group and sulforaphane group (P>0.05).While compared with the cadmium chloride group,the sperm motility rate and sperm total count in sulforaphane and cadmium chloride group were extremely significantly increased (P<0.01),the organ coefficient of testicle and epididymis was increased significantly (P<0.05),the concentration of GSH and activity of T-SOD in testicular tissue were extremely significantly increased (P<0.01),and the concentration of MDA was extremely significantly decreased (P<0.01).The results indicated that sulforaphane had the protection effect on reproduction function of male mice with cadmium poisoning.

Key words: cadmium chloride; sulforaphane; mice; organ coefficient; reproductive function

CLC Number:

R114

GAO Feng, HE Jian-bin, ZHANG Yi, LONG Miao, LI Lin, LI Yan-peng, HAN Yang, WANG Xue, JIANG Li-ying, YANG Shu-hua. Protective Effect of Sulforaphane on Reproductive Function of Male Mice with Cadmium Poisoning[J]. , 2016, 43(2): 471-476.

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Protective Effect of Sulforaphane on Reproductive Function of Male Mice with Cadmium Poisoning

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