酸马奶提取植物乳杆菌DSM20174细菌素的理化特性研究

doi:10.16431/j.cnki.1671-7236.2016.02.023

Abstract

Abstract: To study the physicochemical properties extracted from koumiss of Lactobacillus plantarum DSM20174 bacteriocin,we purified the Lactobacillus plantarum DSM20174 bacteriocins and got the bacteriocin crude extractions after ammonium sulfate precipitation and dialysis.After purification with Sephadex G-100 gel column chromatography,bacteriocin specific activity apparently increased,reaching 154.70 AU/mL,yield was 43.5%.We detected the effects of purified Lactobacillus plantarum DSM20174 bacteriocin on bacteriostatic action of wild bovine pathogenic E.coli O78 by Oxford cup method under 3 different treatments.The results showed that:①In five groups of temperature treatments,the bacteriostatic action decreased with the increase of temperature,bacteriostatic actions of all groups were significantly different (P<0.05).Even after treatment at 121 ℃ 30 min,antimicrobial diameter was 14.90 mm.② After Lactobacillus plantarum DSM20174 bacteriocin were treated with pH 2.0 to pH 12.0,the higher of pH,the smaller of antibacterial diameter.The inhibitory effect of groups were significantly different (P<0.05) except pH 9.0 and pH 10.0.③Lactobacillus plantarum DSM20174 bacteriocins were treated by trypsin,pepsin and papain,antibacterial diameter apparently changed,bacteriostatic action was significantly different (P<0.05) before and after treatment.The results suggested that Lactobacillus plantarum DSM20174 bacteriocins were better antibacterial substances,but not thermal stability substances.It had a relatively wide pH range,and the smaller of pH,the stronger of its antibacterial activity,the antibacterial activity had been enhanced at optimum growth pH.It was not protease stability substances.

Key words: Lactobacillus plantarum DSM20174 bacteriocin; wild bovine pathogenic E.coli O78; antibacterial activity

CLC Number:

S852.4

JIANG Jing, Ao-ri-ge-le, WANG Chun-jie, ZHANG Yan, ZHAO Chen-he, WU Hong-jun, HAO Jian-gang. Study on Physicochemical Properties of Lactobacillus plantarum DSM20174 Bacteriocin from Koumiss[J]. , 2016, 43(2): 444-449.

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Study on Physicochemical Properties of Lactobacillus plantarum DSM20174 Bacteriocin from Koumiss

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