被膜态与浮游态猪源乳杆菌上清液抑菌活性比较分析

doi:10.16431/j.cnki.1671-7236.2019.11.026

Abstract

Abstract: The aim of this study was to compare and analyze the bacteriostatic activity of the supernatants of Lactobacillus johnsonii L-76 and Lactobacillus amylovorus L-102 from swine in biofilm and plankton to pathogenic bacteria.The biofilm formation ability of L-76 and L-102 were measured by crystal violet staining assay.Then the supernatants of Lactobacillus in biofilm and plankton were treated with different influencing factors to study the inhibitory effect of Lactobacillus supernatants on pathogenic bacteria.Scanning electron microscopy was used to observe the effect of supernatant on the morphology of indicator bacteria.The results showed that L-76 and L-102 Lactobacillus had strong ability of biofilm formation and that the growth of pathogenic bacteria was inhibited after co-culture with the supernatant of Lactobacillus in biofilm and plankton.After the action of protease,compared with plankton,the inhibitory zone diameters of biofilm L-76 and L-102 supernatant on the indicator bacteria were significantly reduced.After the action of catalase and different temperatures,there was no significant difference in the antimicrobial activity between L76 and L-102 Lactobacillus supernatants in both conditions (P>0.05).However,they had the best inhibitory effect when pH was 3.0.We observed that the supernatant of biofilm Lactobacillus had a slightly greater effect on the morphology of Staphylococcus aureus by scanning electron microscopy.In conclusion,the supernatant of Lactobacillus contained antimicrobial substances in both conditions,and the activity of bacteriostatic substances in the supernatant of biofilm Lactobacillus was slightly higher or the content was slightly higher.

Key words: swine; Lactobacillus; biofilms; Lactobacillus johnsonii; Lactobacillus amylovorus; antibacterial activity

CLC Number:

S858.28

JIN Shengnan, SHU Huiping, ZHANG Dongxing, ZHAO Zelin, KANG Yuanhuan, ZHANG Lei, SHAN Xiaofeng, QIAN Aidong. Comparative Analysis of the Antimicrobial Activity of Lactobacillus Supernatants from Swine in Biofilm and Plankton[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3358-3369.

References

[1] 陈明亮,蒋勇军,丁雪燕,等.益生菌对猪大肠杆菌病防治作用的研究进展[J].畜牧与兽医,2019,51(4):131-135. CHEN M L,JIANG Y J,DING X Y,et al.Research progress on preventive and therapeutic effects of probiotics on swine Escherichia coli[J]. Animal Husbandry & Veterinary Medicine,2019,51(4):131-135.(in Chinese)
[2] 孙甲午.仔猪大肠杆菌病的诊治技术[J].畜牧兽医科技信息,2015,6:81. SUN J W.Diseases and treatment technologies of Escherichia coli from piglet[J].Chinese Journal of Animal Husbandry and Veterinary,2015,6:81.(in Chinese)
[3] 苏晓轶.猪细菌性腹泻病的临床表现、诊断及防治措施[J].现代畜牧科技,2017,1:52-53. SU X Y.Clinical features,diagnosis and prevention and control measures of bacterial diseases in swine[J].Modern Animal Husbandry Science & Technology,2017,1:52-53.(in Chinese)
[4] 王杨.猪沙门氏菌病的流行病学、鉴别诊断与防控措施[J].现代畜牧科技,2019,5:94-95. WANG Y.Epidemiology,differential diagnosis and control measures of salmonellosis in swine[J].Modern Animal Husbandry Science & Technology,2019,5:94-95.(in Chinese)
[5] MINNQMONQKOLCHAI S,PANBANGRED W.Bacillus probiotics:An alternative to antibiotics for livestock production[J].Journal of Applied Microbiology,2018,124(6):1334-1346.
[6] DAVIS M S,PARROTT T,BROWN D C,et al.Effect of a Bacillus-based direct-fed microbial feed supplement on growth performance and pen cleaning characteristics of growing-finishing pigs[J].Journal of Animal Science,2008,86(6):1459-1467.
[7] WANG A,YU H,GAO X,et al.Influence of Lactobacillus fermentum I5007 on the intestinal and systemic immune responses of healthy and E.coli challenged piglets[J].Antonie Van Leeuwenhoek,2009,96(1):89-98.
[8] GALDEANO C M,LEBLANC A,VINDEROLA G,et al.Proposed model:Mechanisms of immunomodulation induced by probiotic bacteria[J].Clinical and Vaccine Immunology,2007,14(5):485-492.
[9] GARCIA K E,SOUZA T C R,LANDIN G M,et al.Microbial fermentation patterns,diarrhea incidence,and performance in weaned piglets fed a low protein diet supplemented with probiotics[J].Food and Nutrition Sciences,2014,5(18):1776.
[10] CHIAN M L,CHEN H C,CHEN K N,et al.Optimizing production of two potential probiotic Lactobacilli strains isolated from piglet feces as feed additives for weaned piglets[J].Asian-Australasian Journal of Animal Sciences,2015,28(8):1163-1170.
[11] VERT M,DOI Y,HELLWICH K H,et al.Terminology for biorelated polymers and applications (IUPAC Recommendations 2012)[J].Pure & Applied Chemistry,2014,63(11-12):377-410.
[12] SJODIN T.The pH-dependent effect of cationic and non-ionic delmopinol on planktonic and biofilm bacteria[J].Archives of oral biology,2019,102:101-105.
[13] TURCHI B,MANCINI S,FRATINI F,et al.Preliminary evaluation of probiotic potential of Lactobacillus plantarum strains isolated from Italian food products[J].World Journal of Microbiology and Biotechnology,2013,29(10):1913-1922.
[14] STEPANOVIC S,VUKOVIC D,DAKIC I,et al.A modified microtiter-plate test for quantification of staphylococcal biofilm formation[J].Journal of Microbiological Methods,2000,40(2):175-179.
[15] XU Z B,LIU X C,LI L,et al.Development of Staphylococcus aureus enterotoxin in food-borne bacteria[J].Modern Food Science and Technology,2013,29(9):2317-2324.
[16] 李婷,杨舒然,陈敏,等.姜厚朴水提物对大肠杆菌和金黄色葡萄球菌的抑菌机理研究[J].现代食品科技,2016,32(2):84-92. LI T,YANG S R,CHEN M,et al.Antibacterial mechanism of ginger mix-fried magnolia bark extract against Escherichia coli and Staphylococcus aureus[J].Modern Food Science and Technology,2016,32(2):84-92.(in Chinese)
[17] 曾范利,吴胜奎,孙江洋,等.5种中药对大肠杆菌的抑菌作用[J].经济动物学报,2018,3:172-176. ZENG F L,WU S K,SUN J Y,et al.Combined antibacterial effect of five traditional medicines against Escherichia coli[J].Journal of Economic Animal,2018,3:172-176.(in Chinese)
[18] 李晨,张双翔,周碧君,等.腹泻仔猪肠道致病性大肠埃希氏菌和沙门氏菌的分离鉴定与耐药性研究[J].中国畜牧兽医,2017,44(12):3625-3633. LI C,ZHANG S X,ZHOU B J,et al.Isolation,identification and drug resistance analysis of interstinal pathogenic Escherichia coli and Salmonella isolated from diarrhea piglets[J].China Animal Husbandry & Veterinary Medicine,2017,44(12):3625-3633.(in Chinese)
[19] 向红,周藜,廖春,等.金黄色葡萄球菌及其引起的食物中毒的研究进展[J].中国食品卫生杂志,2015,27(2):196-199. XIANG H,ZHOU L,LIAO C,et al.Progress of Staphylococcus aureus and food poisoning[J]. Chinese Journal of Food Hygiene,2015,27(2):196-199.(in Chinese)
[20] 韩乾杰,陈俊杰,王依依,等.益生菌的抑菌物质研究进展[J].饲料博览,2015,8:36-38. HAN Q J,CHEN J J,WANG Y Y,et al.Research progress on antifungal substance of probiotics[J]. Feed Review,2015,8:36-38.(in Chinese)
[21] 杨尚娇.新疆地区干酪中产细菌素乳酸菌的筛选、系统发育及其抑菌活性研究[D].石河子:石河子大学,2015. YANG S J.Bacteriocin-producing lactic acid bacteria isolated from traditional cheese in Xinjiang as potential bio-control agents in food:Isolation,screening and phylogeny[D].Shihezi:Shihezi University,2015.(in Chinese)
[22] 罗宝龙,魏军林,黄丽丽,等.猪源产细菌素芽孢杆菌的筛选及抑菌特性[J].微生物学通报,2018,45(6):1342-1349. LUO B L,WEI J L,HUANG L L,et al.Selection and antibacterial activity of bacteriocin-producinng Bacilli from swine[J]. Microbiology China,2018,45(6):1342-1349.(in Chinese)
[23] 黎杰.马奶酒中产细菌素乳酸菌的筛选及细菌素理化特性研究[D].呼和浩特:内蒙古农业大学,2015. LI J.Screening of the bacteriocin-producing lactic acid bacteria from kumiss and study on physicochemical properties of bacteriocin[D].Hohhot:Inner Mongolia Agricultural University,2015.(in Chinese)
[24] 徐志娇,韩晓江,宋子涵,等.一株植物乳杆菌对脂环酸芽孢杆菌的抑菌活性[J].西北农林科技大学学报(自然科学版),2019,47(6):124-131. XU Z J,HAN X J,SONG Z H.Antibacterial activity of a Lactobacillus plantarum strain against Alicyclobacillus acidoterrestris[J].Journal of Northwest A & F University (Natural Science Edition),2019,47(6):124-131.(in Chinese)

Comparative Analysis of the Antimicrobial Activity of Lactobacillus Supernatants from Swine in Biofilm and Plankton

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