青海高原牦牛PRDM16基因克隆、生物信息学及组织差异表达分析

doi:10.16431/j.cnki.1671-7236.2016.02.011

Abstract

Abstract: In this study,the part coding region of PRDM16 gene of the Qinghai plateau yak was cloned and its bioinformatics and differential expression of PRDM16 gene of muscle tissue were analyzed in different sex individual.The PRDM16 gene part of CDS region was cloned from the longissimus dorsi muscle tissue of Qinghai plateau yak,analyzed its characteristics of bioinformatics and detected gene expression levels of different sex individuals in muscle tissue by Real-time PCR.The results showed that the sequence length of the cloned fragment was 323 bp,the homology between Qinghai plateau yak and cattle was 100%.It encoded 99 amino acids,containing domains of MDS1-EVI1 (complex locus protein MDS1) family proteins function,CATH protein function and typical structure of the curly spiral.PRDM16 protein was 20% of the similarity with people methyltransferase protein domain PR protein 1.PRDM16 gene expression of female yak was significantly higher than male yak's in longissimus dorsi muscle tissue (P<0.01).The test results provided not only research foundation for genetic progress but also technical references for yak meat quality analysis.

Key words: Qinghai plateau yak; PRDM16 gene; longissimus dorsi; gene expression

CLC Number:

ZHAO Sheng-jun, ZHANG Yong, GUO Xian. Cloning,Bioinformatics and Tissue Differential Expression Analysis of PRDM16 Gene in Qinghai Plateau Yak[J]. , 2016, 43(2): 363-370.

References

[1] 孔祥颖,张丽,保善科,等.放养过程中补饲对青海高原牦牛产肉能力及肉品质的影响[J].中国畜牧兽医,2015,42(1):104-108.
[2] Huang X D,Tan H Y,Long R,et al.Comparison of methanogen diversity of yak (Bos grunniens) and cattle (Bos taurus) from the Qinghai-Tibetan plateau,China[J].BMC Microbiology,2013,12:237-247.
[3] 杨雪蓉,陈代文,余冰,等.PRDM16的研究进展[J].动物营养学报,2010,22(6):1477-1481.
[4] Hohenauer T,Moore A W.The prdm family:Expanding roles in stem cells and development[J].Development,2012,139(13):2267-2282.
[5] Kajimura S,Seale P,Tomaru T,et al.Regulation of the brown and white fat gen programs through a PRDM16/CtBP transcriptional complex[J].Genes Dev,2008,22(10):1397-1409.
[6] Cristancho A G,Schupp M,Lefterova M I,et al.Repressor transcription factor 7-like 1 promotes adipogenic competency in precursor cells[J].Proc Nati Acad USA,2011,108(39):16271-16276.
[7] Lee J E,Ge K.Transcriptional and epigenetic regulation of PPARγ expression during adipogenesis[J].Cell Biosci,2014,4:29-39.
[8] Chuikov S,Levi B P,Smith M L,et al.PRDM16 promotes stem cell maintenance in multiple tissues,partly by regulating oxidative stress[J].Natural Cell Biology,2010,12(10):999-1006.
[9] Yoshida M,Nosaka K,Yasunaga J,et al.Aberrant expression of the MEL1S gene identified in association with hypomethylation in adult T-cell leukemia cells[J].Blood,2004,103(7):2753-2760.
[10] DiZazzo E,De Rosa C,Abbondanza C,et al.PRDM proteins:Molecular mechanisms in signal transduction and transcriptional regulation[J].Biology,2013,2(1):107-141.
[11] Herz H M,Garruss A,Shilatifard A.SET for life:Biochemical activities and biological functions of SET domain-containing proteins[J].Trends in Biochemical Sciences,2013,38(12):621-639.
[12] Lanouette S,Mongeon V,Figeys D,et al.The functional diversity of protein Iysine methylation[J].Molecular Systems Biology,10(4):724-749.
[13] Farmer S R.Brown fat and skeletal muscle:Unlikely cousins[J].Cell,2008,134(5):726-727.

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Cloning,Bioinformatics and Tissue Differential Expression Analysis of PRDM16 Gene in Qinghai Plateau Yak

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