云南蓝舌病病毒1型毒株M6基因序列分析

doi:10.16431/j.cnki.1671-7236.2016.02.008

Abstract

Abstract: The object of this study was to investigate the evolution and variation of NS1 encoding gene (M6) of Bluetongue virus 1 (BTV-1) from Yunnan province and the evolutionary relationship of strains which from Yunnan province and other countries.RNA were extracted from four strains (Y863,SZ120169,6-12 and 7-12),and M6 gene were amplified by using specific primer and sequenced,which were analyzed by using bioinformatics software for nucleotides homology and phylogenetic relationships.The results showed that four strains M6 gene were 1 763 bp;The homology of nucleotides among four strains M6 gene were 95.2% to 99.9%,the amino acids among four strains M6 gene were 97.6% to 99.8%,the homology of nucleotides between Y863 (1979) and 3 strains (SZ120169,6-12 and 7-12) were 95.5%,95.2% and 95.2%,the amino acids between Y863 (1979) and 3 strains were 97.6%,98.4% and 98.2%,the homology of nucleotides and amino acids were high (96.9% to 99.9% and 99.1% to 99.8%,respectively) among four Yunnan strains.BTV was divided into two clusters (Western and Eastern) and four strains (BTV-1) from Yunan province belong to Eastern cluster.The homology of nucleotides and amino acids among four Yunnan strains was 95.2% to 99.9% and 97.6% to 99.8% respectively;The genetic distance were close among four Yunnan strains and strains from Greece and Australia,the homology of nucleotides and amino acids between them were 90.4% to 95.6% and 95.1% to 99.1%;The genetic distance were distinct among four Yunnan strains and strains from Mediterranean countries (Italy,Fance,Algeria,Morocco and Tunisia) and South Africa;The homology of nucleotides and amino acids between them were below 83.8% and 95.7%,so we found that gene clusters distribution was related to the geographical distribution for BTV.In a conclusion,four Yunnan strains belong to Eastern cluster and the speed of genetic variation of M6 from Yunnan province was slow,so M6 gene could be used in study of gene group of distribution and origin of geographical area.

Key words: BTV; M6 gene; sequence analysis

CLC Number:

S852.65⁺9.4

LI Nan, ZHU Jian-bo, XIAO Lei, LI Le, YANG Heng, MENG Jin-xin, HE Yu-wen, LI Hua-chun. Sequence Analysis of Bluetongue Virus 1 M6 Gene in Yunnan Province[J]. , 2016, 43(2): 340-347.

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Sequence Analysis of Bluetongue Virus 1 M6 Gene in Yunnan Province

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