H6N1亚型禽流感病毒二重荧光RT-PCR检测方法的建立

doi:10.16431/j.cnki.1671-7236.2016.02.006

Abstract

Abstract: According to the sequences of HA and NA genes of H6 and N1 subtype avian influenza virus (AIV),two pairs of specific primers and two TaqMan probes with different fluorescence were designed.The duplex Real-time RT-PCR assay was developed and optimized to simultaneously detect H6 and N1 subtypes AIV in one reaction.The result showed that the specificity of this assay was high and only amplified H6 and N1 subtypes AIV,and was not cross-reactive with other H and N subtypes AIV,newcastle disease virus and infectious bronchitis virus.The detection limit of this assay was 100 copies/μL of H6N1 subtype AIV.This newly developed duplex Real-time RT-PCR assay was a rapid,specific and sensitive method for the detection of H6N1 subtype AIV,and it could provid a technical support to prevent and control H6N1 subtype AIV.

Key words: avian influenza virus; H6N1 subtype; duplex Real-time RT-PCR

CLC Number:

LUO Si-si, XIE Zhi-xun, XIE Zhi-qin, DENG Xian-wen, LIU Jia-bo, XIE Li-ji, HUANG Li, HUANG Jiao-ling, ZENG Ting-ting, ZHANG Yan-fang, WANG Sheng. Establishment of a Duplex Real-time RT-PCR for Detection of H6N1 Subtype Avian Influenza Virus[J]. , 2016, 43(2): 326-332.

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Establishment of a Duplex Real-time RT-PCR for Detection of H6N1 Subtype Avian Influenza Virus

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