1~35日龄羔羊小肠消化酶活性的研究

doi:10.16431/j.cnki.1671-7236.2015.12.013

Abstract

Abstract: 56 Small-tail Han sheep lambs with birth body weight (3.38±0.33)kg were randomly divided into two groups (n=28 each group),fed with milk powder substitute or fish meal substitute since 4 days of age respectively and after 21 days of age,eight lambs in each group were turned to starting feed.At the age of 7,14,21,28 and 35 days,four lambs were slaughtered from each group respectively.The contents of small intestine were collected,determining the α-amylase activity,lactase activity,trypsinase activity,chymotrypsin activity of chyme supernatant from the small intestine,to investigate the enzyme activity in small intestine at different ages with different milk substitutes.The results showed that the activity of α-amylase,lactase,trypsinase,chymotrypsin of chyme supernatant from small intestine of the lamb fed with milk powder substitute was linearly correlated with age,and the average daily gain was 157.2 g/d,without significant change with age.At different ages,the activity of α-amylase,lactase,trypsinase,chymotrypsin of chyme supernatant from small intestine of the lambs fed with fish meal substitute was inferior to those fed with milk powder substitute,43.7%,46.0%,35.2% and 10.2% on average,respectively,and the average daily gain was reduced,especially after 21 days of age.It was revealed that the growth of the lamb fed with fish meal substitute might be influenced by the immune response of fish meal protein.After the lambs were given starting feed,the activity of α-amylase,lactase,trypsinase,chymotrypsin of chyme supernatant from small intestine decreased,but recovered later.The activity of α-amylase,lactase,trypsinase of the lamb fed with milk powder substitute before 21 days of age reduced more than the lambs fed with fish meal substitute,but the activity was still high.It was concluded that the activity of α-amylase,trypsinase,chymotrypsin of chyme supernatant from small intestine of the lamb increased with age,the activity of lactase decreased with age,while the activity of digestive enzyme in the small intestine of the lamb fed with fish meal substitute was significantly reduced.After the lambs were fed with starter,digestive obstacle occurred,but disappeared later.

Key words: lamb; small intestine; digestive enzyme; development

CLC Number:

S826

LI Feng-ming, LUO Qiu-jiang, ZANG Chang-jiang, PAN Rong, LI Wei-dong. Study on Development of the Small-intestinal Digestive Enzyme Activity of 1-35 days' Small-tail Han Sheep Lambs[J]. , 2015, 42(12): 3192-3201.

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Study on Development of the Small-intestinal Digestive Enzyme Activity of 1-35 days' Small-tail Han Sheep Lambs

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