五指山猪FUT1基因SNP检测及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2015.11.015

Abstract

Abstract: This study was aimed to detect FUT1 gene,analyze its structure,and reveal the distribution of SNP in Wuzhishan pig.FUT1 gene was amplified by PCR.Then the gene mutation was identified by sequencing.PCR-RFLP method was used to detect the FUT1 gene of 120 Wuzhishan pigs.Some characters of FUT1 gene were analyzed by bioinformatics tools.Two mutations were detected in the FUT1 genomic fragment,one in the coding region (A+307G) and the other in the 3'regulatory region (A+1856C).At the A+307G locus of coding region in FUT1 gene,100% of Wuzhishan pigs were GG genotype.In addition,bioinformatics analysis revealed that FUT1 gene promoter and the second exon region contained CpG islands.In this study,we obtained the sequences of FUT1 gene which laid a foundation for further studying its functions in Wuzhishan pig.

Key words: Wuzhishan pig; FUT1 gene; polymorphism; bioinformatics analysis

CLC Number:

CHAO Zhe, WANG Feng, HUANG Li-li, LIU Hai-long, SUN Rui-ping, ZHENG Xin-li. SNP Detection and Bioinformatics Analysis of FUT1 Gene of Wuzhishan Pig[J]. , 2015, 42(11): 2915-2920.

References

[1] 吴延军,覃金,顾祖华,等.广西巴马小型猪FUT1基因的克隆和序列分析[J].黑龙江畜牧兽医,2014,7:70-72.
[2] 周利华,舒青龙,彭秋玲,等.FUT1基因新cSNPs的鉴别及其对仔猪抗大肠杆菌F18侵染的影响[J].中国农业科学,2011,44(8):1720-1726.
[3] Mainil J G,Jacquemin E,Pohl P,et al.DNA sequences coding for the F18 fimbriae and AIDA adhesion are localized on the same plasmid in Escherichia coli isolates from piglets[J].Vet Microbiol,2002,86(4):303-311.
[4] Meijerink E,Fries R,Vogeli P,et al.Two α(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (s) and Escherichia coli F18 receptor (ECF18R) loci[J].Mamm Genome,1997,8(10):736-741.
[5] Yan X M,Ren J,Guo Y M,et al.Research on the genetic variations of α1-fucosyltransferase (FUT1) gene in 26 pig breeds[J].Acta Genet Sin,2003,30(9):830-834.
[6] 晁哲,王峰,魏立民,等.海南五指山猪Myf5基因克隆与序列分析[J].黑龙江畜牧兽医,2014,5:71-73.
[7] 陈克研,刁玉刚,周锦,等.三种麻醉方法在巴马小型猪失血性休克实验中的麻醉效果比较[J].中国比较医学杂志,2013,23(10):53-57.
[8] Murayama N,Kaneko N,Horiuchi K,et al.Cytochrome P450-dependent drug oxidation activity of liver microsomes from Microminipigs,a possible new animal model for humans in non-clinical studies[J].Durg Metab Pharmacokinet,2009,24(4):404-408.
[9] Stembirek J,Buchtova M,Kral T,et al.Early morphogenesis of heterodont dentition in minipigs[J].Eur J Oral Sci,2010,118(6):547-558.
[10] Helke K L,Swindle M M.Animal models of toxicology testing:The role of pigs[J].Expert Opin Drug Metab Toxicol,2013,9(2):127-139.
[11] 商海涛,魏泓.我国小型猪品系资源状况初浅分析[J].中国实验动物学报,2007,15(1):70-75.
[12] 曲娟娟,于敏,董蕾,等.SNP在动植物遗传育种中的应用[J].东北农业大学学报,2011,42(12):9-13.
[13] 张引红.中外六个猪种FUT1基因多态性研究[J].中国比较医学杂志,2007,17(8):487-489.
[14] Mizuguchi Y,Takizawa T,Uchida E.Host cellular microRNA involvement in the control of hepatitis B virus gene expression and replication[J].World J Hepatol,2015,7(4):696-702.
[15] 邱峥艳,郭将,杨春,等.DNA甲基化在动物遗传育种中的研究进展[J].中国畜牧兽医,2012,39(7):173-178.
[16] Hyde S C,Pringle I A,Abdullah S,et al.CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression[J]. Nat Biotechnol,2008,26(5):549-551.
[17] Meng H,Cao Y,Qin J,et al.DNA methylation,its mediators and genome integrity[J].Int J Biol Sci,2015,11(5):604-617.

SNP Detection and Bioinformatics Analysis of FUT1 Gene of Wuzhishan Pig

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[2]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[3]	ZHUO Na, ZHU Zhongwu, LI Jing, HU Shixiang, WANG Jianchang, LIN Hua, CHEN Chaolin, HAN Diangang, AI Jun, CHEN Peifu. Bioinformatics Analysis and Eukaryotic Expression of ORF2 Protein of Swine Hepatitis E Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1959-1970.
[4]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[5]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[6]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[7]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Bioinformatics Analysis and Prokaryotic Expression of EGR-07243 and EGR-07243 Genes of Echinococcus granulosus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 847-858.
[8]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[9]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[10]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[11]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[12]	TANG Jingyu, DU Hanyu, MENG Chunchun, LIU Guangqing. Cloning,Bioinformatics Analysis and Subcellular Localization of Interferon-stimulated Gene 15 in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2843-2854.
[13]	FU Yongzhi, MA Xiaojun, WANG Maohan, LI Zhe, CHEN Zhiyuan, QUAN Wei, ZHAO Han, SU Yongmei, WU Jianhua, ZHU Wenjin. Bioinformatics Analysis and Tissue Expression of MSTN Gene in Yangyuan Donkeys [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2910-2919.
[14]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.
[15]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.