贵州省山羊源流产细菌调查及致病机理初探

doi:10.16431/j.cnki.1671-7236.2015.10.039

Abstract

Abstract: In order to study the main reason of bacterial goat abortion in Guizhou province,and analyze the bacteria impact of abortion goat immune balance in normal pregnancy animals,107 samples of the goat fetus,uterus and vaginal swabs were collected from clinical health and morbidity abortion goat in 12 scale goat farms of 6 areas in Guizhou province.The bacteria were isolated and identified.The drug susceptibility disc agar diffusion method was launched for the survey of the resistance of three main sources of goat isolates to 20 kinds of antibiotics.The BALB/c mice model of abortion was established using abortion bacteria isolated from goats.The pregnancy related hormones and cytokines were determined by ELISA method.The results showed that 302 strains bacteria of 13 genera were isolated from the samples,and the species of bacteria isolated from morbidity abortion goat were more than the clinical health goats.The bacteria resistant rate of goat reproductive organs in abortion goats was higher than that of clinical healthy goats bacteria.The varying degrees of abortion of BALB/c mice could cause by Escherichia,Staphylococcus and Streptococcus isolated from goats,the rates were Escherichia>Staphylococcus>Streptococcus,respectively and the embryo resorption rates were Escherichia>Staphylococcus>Streptococcus,respectively.Compared with the control group,IFN-γ and IL-2 in the serum were increased;However,IL-4,IL-10 and progesterone were decreased.The results indicated that the occurrence of bacterial goat abortion diseases had certainly related to the number of bacterial types and the level of drug resistance in goat reproductive organs.The levels of pregnancy related hormones and cytokines in goats serum could be changed by abortion bacteria,thus lead to goats abortion.

Key words: goat; bacteria; resistance; cytokines; abortion

CLC Number:

S852.61

WANG Wei, WANG Kai-gong, ZHOU Bi-jun, WANG Ming, FENG Xu-fang, MA Guang-qiang, LUO Yi. Preliminary Study on Abortion Bacteria from Goat in Guizhou Province and their Pathogenic Mechanism[J]. , 2015, 42(10): 2779-2787.

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Preliminary Study on Abortion Bacteria from Goat in Guizhou Province and their Pathogenic Mechanism

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