7种家养动物全基因组微卫星分布的差异研究

doi:10.16431/j.cnki.1671-7236.2015.09.031

Abstract

Abstract: To investigate whether difference in SSRs distribution of 7 domestic animals genomes, the microsatellite sequence were searched by using MSDB (Microsatellite Search and Building Database) and analyzed in pig, horse, cow, goat, sheep, chicken and dog genomes.Preliminary results showed that SRRs number, frequency and density were found to be largely variable among these animals.The most abundant distribution was observed in dog genome with 1 436 242 identified SSRs loci.The minimum SSRs (276 564) number was found in chicken genome.Yet, the minimum SSRs (430 760) frequency and density were found in horse genome.Among these SSRs, mononucleotide repeat type motif was the most abundant and pentanucleotide repeat type motif was the rarest in all animals.Meanwhile, all the dominant SSRs for different repeat types were abundant in nucleotide A and T.In addition, there was variability among 7 animals of SSRs distribution.In general, SSRs distribution was the most similar in cow, goat and sheep genomes, and chicken was the most different from other animals.The results also showed that the length of microsatellite ranged mainly from 12 to 20 bp in all animals, which might be due to selection pressure of convergence.In short, these results revealed difference as well as conservation in SSRs distribution among different domestic animals genomes.In particular, genetically close animals tended to have similar SSR distribution patters.This would provide a basis for subsequent research of SSRs function.

Key words: microsatellite; repeat type; repeat copy category; SSRs length

CLC Number:

S813

WANG Yue-yue, LIU Xue-xue, DONG Kun-zhe, CHEN Xiao-fei, YE Shao-hui, MA Yue-hui. Distribution Difference of Microsatellite in 7 Domestic Animals Genomes[J]. , 2015, 42(9): 2418-2426.

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Distribution Difference of Microsatellite in 7 Domestic Animals Genomes

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