牛结核分枝杆菌实时荧光定量PCR检测方法的建立及应用

doi:10.16431/j.cnki.1671-7236.2015.08.009

Abstract

Abstract: To develop a detection method for rapid diagnosis of dairy cow tuberculosis, evaluation of the raw milk contamination status and tracing the route of transmission, specific primers of Mycobacterium bovis were designed to develop the FQ-PCR assay and the reaction conditions were optimized.Standard curve of the FQ-PCR test was developed and its properties were evaluated.The results showed that the developed FQ-PCR test could be used to detect the Mycobacterium bovis.The best primer concentration and annealing temperature were 400 nmol/L and 52 ℃, respectively.Properties evaluation showed that the method had good specificity, sensitivity, repeatability and clinical application.The test results indicated this method could be used to qualitatively and quantitatively detect Mycobacterium bovis.It would be an important technology for diagnosis and decontamination of dairy cow tuberculosis and safety assessment of raw milk.

Key words: Mycobacterium bovis; FQ-PCR; development; application

CLC Number:

S858.23

CHENG Zhen-tao, YUE Jun, ZHANG Hua, ZHOU Bi-jun, WEN Ming, WANG Kai-gong, OU De-yuan, QIN Lan, XU Chun-zhi. Development and Application of FQ-PCR Assay for Detecting Mycobacterium bovis[J]. , 2015, 42(8): 1979-1985.

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Development and Application of FQ-PCR Assay for Detecting Mycobacterium bovis

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