一株临床2型猪链球菌的分离鉴定及生物学特性分析

doi:10.16431/j.cnki.1671-7236.2015.07.040

Abstract

Abstract: In order to determine the pathogen and its virulence characteristics of a suspected case of piglets streptococcosis in a pig farms in Jiangxi province during September to November of 2013,a strain was isolated from the joint fluid and cerebrospinal fluid of one diseased piglet.The isolated strain was identified by culture characteristics,biochemical test,16S rDNA sequence analysis,serotype PCR test and further studied the presence of the following virulence genes:Glutamate dehydrogenase (gdh),virulent-assocciated sequence (orf2),fibronectin-binding proteins (fbps),suilysin (sly),extracellular protein factor (epf),muramidase-released protein (mrp) and antibiotic sensitivity tests.The isolated strain was identified as Streptococcus suis serotype 2 by culture characteristics,biochemical test,16S rDNA sequence analysis and serotype PCR test,and it was named as JMS2. Detection results of virulence gene showed that the distribution of main virulence genes of JMS2 were gdh⁺/orf2⁺/fbps⁺/sly^-/epf^-/mrp^-,suggesting that the strain maybe not likely to be high virulence.Drug sensitivity tests showed that the JMS2 was susceptibility to β-lactams such as penicillin,amoxicillin,but resistant to azithromycin,SMZ-TMP,and so on.The result was significant for the clinical prevention and treatment of swine streptococcosis.

Key words: Streptococcus suis serotype 2; isolation and identification; virulence gene; drug sensitivity test

CLC Number:

S852.6

FU Meng-yu, LIN Li-ping, GUO Dao-chu, ZHANG Feng-ying, WU Guo-ping. Isolation,Identification and Biological Characteristics Analysis of a Strain of Clinical Streptococcus suis Serotype 2[J]. , 2015, 42(7): 1897-1904.

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Isolation,Identification and Biological Characteristics Analysis of a Strain of Clinical Streptococcus suis Serotype 2

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