成都某种禽场鸡胚沙门氏菌的检测

doi:10.16431/j.cnki.1671-7236.2015.06.037

Abstract

Abstract: In a poultry farm of Chengdu city,Sichuan province,isolation,identification and drug sensitivity test of Salmonella were conducted from 156 dead embryos.In this experiment,we used Salmonella chromogenic medium,biochemical identification of enterobacteriaceae,trisaccharide iron experiment,Salmonella polyvalent serum and 16S rRNA PCR to identify suspected strains,PCR identification of isolated Salmonella were conducted with 6 kinds of virulence genes.The results concluded that Salmonella separation rate was 15.4%(24/156),including Salmonella typhi accounted for 58.3%(14/24); Detection rates of fimY,invA and mgtC virulence genes were all 100%;The isolated Salmonella were resistant to most clinical drugs to Salmonella.

Key words: dead embryo; isolation; identification; virulence genes; drug sensitivity

CLC Number:

S852.61

ZHANG Li-fang, XIAO Qiao, LUO Wei. Detection of Salmonella in Chicken Embryos in a Breeding Poultry Field of Chengdu[J]. , 2015, 42(6): 1571-1579.

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Detection of Salmonella in Chicken Embryos in a Breeding Poultry Field of Chengdu

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