Abstract: The aim of this study was to investigate the association between CARD15 gene StyⅠpolymorphism and susceptibility to tuberculosis (TB) of dairy cows from Yunnan plateau.A total of 201 dairy cows (103 cases and 98 controls) from Kunming,Yuxi and Dali were used in this study.We designed a specific primer pair for PCR-RFLP analysis.By using PCR-RFLP genotyping and PCR sequencing,We analysized allele frequency and correlation between polymorphism of CARD15 gene and susceptibility to bovine tuberculosis in Yunnan province.The results showed that there were three genotypes in Sty Ⅰ enzyme site,AA,AG and GG.In cases,the genotype frequencies of AA,AG and GG were 0.03,0.42 and 0.55,respectively,while in controls were 0.01,0.05 and 0.94,and the allele frequencies of A and G were 0.24 and 0.76 in cases,while were 0.04 and 0.96 in controls.Then analysizing the genetic pattern of polymorphic loci,we found that the best genetic model was dominant and the frequency of AG-AA in cases (44.7%) was apparently higher than that in controls (6.1%) (OR(95% CI)=0.08 (OR< 1)),so extremely significant difference existed between cases and controls (P< 0.0001).The polymorphism of StyⅠenzyme cut site in CARD15 gene was significantly associated with the susceptibility to tuberculosis of Yunnan plateau dairy cows,and AG-AA might be the susceptible genotype to tuberculosis.

Key words: CARD15 gene; dairy cows; tuberculosis; single nucleotide polymorphism