通过优化超排后合笼时间高效获取小鼠孵化囊胚

doi:10.16431/j.cnki.1671-7236.2015.05.033

Abstract

Abstract: The persent study was aimed to investigate the optimization of mice cage time after superovulation, and efficient acquisition of mouse hatched blastocysts.The experiment selected 150 ICR female mice aged 8 weeks, were randomly divided into 5 groups, the same time superovulation treatment according to 1:1, after the male and female in 18:00, 19:00, 20:00, 21:00 alloy cage overnight, the next day as early as 08:00 check, found that vaginal suppository for the first day of the pregnancy (D1).Take the pregnant the fifth day (D5) mice were sacrificed, their bilateral uterine horns, rushed from the embryonic.Statistics each thrust ratio and the total number of embryos hatched blastocysts/take not hatched blastocysts, was used as the index to evaluate embryos to obtain efficiency;Statistical trophectoderm cell number/inner cell mass number, as a reference index to evaluate the quality of embryo.The results found that groups of Ⅰ, Ⅱ and Ⅲ were no significant difference in the blastocyst number (P>0.05), but there was increasing trend, group Ⅳ was significantly higher than the other three groups (P<0.05).Groups Ⅰ, Ⅱ, Ⅲ and Ⅳ within the cell mass cells number/trophectoderm cell number were 23.18%, 23.55%, 21.72% and 23.28%, there was no significant difference in each group (P>0.05).The results showed that the corresponding set of Ⅳ cage got the time of mouse hatched blastocysts to obtain the highest efficiency, there was no significant differences in embryonic blastocyst quality.

Key words: mouse; hatched blastocysts; superovulation; cage time

CLC Number:

S814.8

ZHAI Chun-dong, GU Mei-chao, LIU Di, WANG Li-hong, QI Xiao-long, ZHANG Jia-nan, LIU Yun-hai, NI He-min, GUO Yong. Study on Time Period Model for Efficiently Collecting Hatched Blastocysts Flushed from Murine Uterus[J]. , 2015, 42(5): 1253-1258.

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Study on Time Period Model for Efficiently Collecting Hatched Blastocysts Flushed from Murine Uterus

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