H5N1亚型禽流感病毒感染海兰白鸡模型的建立

doi:10.16431/j.cnki.1671-7236.2015.03.028

Abstract

Abstract: The study was aimed to establish the suitable model for Hy-line Variety White chickens infected by H5N1 subtype avian influenza virus (AIV).The AIV used in this study was A/goose/guangdong/1/96 (H5N1) (GD1/96) virus,which was saved a goose source of highly pathogenic AIV,egg infectious dose (EID₅₀) and median lethal dose (LD₅₀) of 4-week-old Hy-line Variety White chickens were detected.In the infection model study,thirty 4-week-old chickens were randomly divided into three groups with 10 chickens in each group,5 direct infection,five cohabitation,the virus was diluted to 10^4.5 EID₅₀,the infection chicken were inoculated with 0.1 mL to nasal,eye each,the control chicken received PBS,24 h after infecting the cohabitation chicken were added; clinical signs were assessed daily for 14 d post-infection,the time of death were record,throat swabs and cloacal swabs were collected daily,necropsy of three chickens was performed on 3 and 5 d post infection and cohabitation,the trachea,lung,brain,spleen,kidney and duodenum were collected for virus isolation.The expression of IFN-α and TNF-α in 3 and 5 d post infection and cohabitation was qualified by qRT-PCR.The results showed that the EID₅₀ and LD₅₀ of 4-week-old Hy-line Variety White chickens were 10^-8.167/0.1 mL and 10^4.5 EID₅₀,respectively.The infection model study showed that all chickens died 8 d after infection with 10^4.5 EID₅₀ of the infection dosage,3 d post infection and cohabitation,high titers of AIV were detected in chicken throat swabs and cloacal swabs; 6 kinds of tissues in 3 and 5 d post infection and cohabitation were isolated to be high titer virus; the expression of IFN-α and TNF-α in lung tissue were significantly increased in infected chickens and cohabitation group (P <0.05).The model for Hy-line Variety White chickens infected by H5N1 subtype AIV was established.This study would lend some references for study of the pathogenic mechanism of H5N1 subtype AIV and generation of anti-influenza transgenic chickens against influenza virus research.

Key words: avian influenza virus; H5N1; Hy-line Variety White chicken; pathogenicity; infection model

CLC Number:

S852.65

WANG Wei, ZHANG Ya-chun, DENG Rui-po, LI Yue, ZHAO Ying-hui, CHEN Hong-yan, MENG Qing-wen. Establishment of Model for Hy-line Variety White Chickens Infected by H5N1 Subtype Avian Influenza Virus[J]. , 2015, 42(3): 690-695.

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Establishment of Model for Hy-line Variety White Chickens Infected by H5N1 Subtype Avian Influenza Virus

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