动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定

doi:10.16431/j.cnki.1671-7236.2015.02.035

Abstract

Abstract: The assay was aimed to investigate prevalent Salmonella strains from animal,their antibiotic susceptibility,and distribution of florfenicol resistant genes in Salmonella isolates with resistance to florfenicol.Pathogens were isolated from clinical samples with suspected salmonellosis,and identified by mPCR.Antibiotic resistance to 23 antibiotics was determined by antibiotic susceptibility test according to the K-B method.The isolates with resistance to florfenicol were chosen to PCR amplification for floR,fexA,fexB,cfr and pexA genes.The results showed 61 Salmonella strains were isolated,and the numbers of S.Enteritidis,S.Pullorum and S.Typhimurium were 10,12 and 39,respectively.All strains were resistant to penicillin,erythromycin and vancomycin,and 90.16% of the isolates were resistant to six or more than six actibiotics.The floR genes were detected in 8 out of 12 florfenicol-resisitant S.Typhimurium strains,and no other genes were detected.These data indicated that S.Typhimurium was the dominant serotype of Salmonella isolated from geese.The floR gene played a major role in its florfenicol resistance.However,it might exist other antibiotic resistance mechanism.

Key words: Salmonella; serotype; antibiotic resistance; floR gene

CLC Number:

S852.61⁺4

HUANG Kai, CHEN Su-juan, HUANG Jun, YANG Lin, JU Yong, ZHU Xiang-ru, SUN Zhi-hao, PENG Da-xin. Analysis of Antibiotic Resistance of Salmonella Isolated from Animals and Identification of its Florfenicol Resistant Gene[J]. , 2015, 42(2): 459-466.

References

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Analysis of Antibiotic Resistance of Salmonella Isolated from Animals and Identification of its Florfenicol Resistant Gene

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