摩拉水牛和尼里-拉菲水牛PRKAA2基因SNPs检测及遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2018.05.019

摘要/Abstract

摘要：

为研究水牛蛋白激酶AMP活化的催化亚基α2（protein kinase AMP-activated catalytic subunit alpha 2，PRKAA2）基因多态性，本试验以摩拉水牛和尼里-拉菲水牛基因组DNA为模板，扩增PRKAA2基因外显子4及内含子3部分序列，通过常规测序法检测其SNP并进行遗传多样性分析。结果发现，PRKAA2基因外显子4内存在1个SNP位点（c.462 G>A），PRKAA2基因内含子3部分序列存在3个SNPs位点（IVS3.557 T>C、IVS3.560 C>T和IVS3.565 G>A）。经遗传多样性分析表明，在c.462 G>A位点的野生纯合型和杂合型比突变纯合型更有优势，IVS3.557 T>C和IVS3.560 C>T位点的突变纯合型为非优势基因型，IVS3.565 G>A位点杂合型为优势基因型。IVS3.565 G>A位点在摩拉水牛群体中处于Hardy-Weinberg非平衡状态；c.462 G>A位点在尼里-拉菲水牛群体中处于Hardy-Weinberg非平衡状态。4个SNPs位点在摩拉水牛群体中均为中度多态；c.462 G>A、IVS3.557 T>C位点在尼里-拉菲水牛群体中为低度多态，IVS3.560 C>T、IVS3.565 G>A位点为中度多态。IVS3.557 T>C位点在两个水牛群体中杂合度较低。说明摩拉水牛IVS3.565 G>A位点和尼里-拉菲水牛c.462 G>A位点的基因型频率和基因频率遗传状态不平衡，尼里-拉菲水牛群体中IVS3.557 T>C位点遗传变异小，选择潜力不高。4个多态位点可以构建5种单倍型，其中T-C-G-G是摩拉水牛群体和尼里-拉菲水牛群体的优势单倍型。综上，本研究检测的摩拉水牛和尼里-拉菲水牛PRKAA2基因上4个SNPs位点可为水牛标记辅助选择育种提供参考。

关键词: 摩拉水牛; 尼里-拉菲水牛; PRKAA2基因; SNP; 常规测序

Abstract:

In order to study the polymorphism of protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2),the genome DNA of Murrah and Nili-Ravi buffaloes were used as templates to amplify exon 4 and intron 3 partial sequences of PRKAA2 gene,the SNPs were detected by routine sequencing,and the genetic diversity was analyzed.The results showed that there was 1 SNP (c.462 G>A) in exon 4 and 3 SNPs (IVS3.557 T>C,IVS3.560 C>T and IVS3.565 G>A) in the intron 3.The genetic diversity results showed that wild homozygote and heterozygote were more advantages than mutant homozygote at c.462 G>A,the mutation homozygous genotypes were non-dominant genotypes at IVS3.557 T>C and IVS3.560 C>T,and the heterozygous genotypes were dominant genotypes at IVS3.565 G>A.IVS3.565 G>A in Murrah buffaloes and c.462 G>A in Nili-Ravi buffaloes were in non-equilibrium.All of 4 SNPs in Murrah buffaloes were at moderate polymorphism,c.462 G>A and IVS3.557 T>C in Nili-Ravi buffaloes were at low polymorphism,and IVS3.560 C>T and IVS3.565 G>A were at moderate polymorphism.The heterozygosity of IVS3.557 T>C was lower in two buffalo populations.The genotype frequency and gene frequency of IVS3.565 G>A in Murrah buffalo and c.462 G>A in Nile-Rafi buffalo were imbalanced,the genetic variation of IVS3.557 T>C in Nile-Rafi buffalo was low,without a high selection potential.4 SNPs would be combined into 5 haplotypes,T-C-G-G type was the dominant haplotype in two buffalo populations.In conclusion,4 SNPs of PRKAA2 gene in Murrah and Nili-Rafi buffaloes could provide a reference for marker-assisted selection breeding.

Key words: Murrah buffalo; Nili-Ravi buffalo; PRKAA2 gene; SNP; routine sequencing

中图分类号:

S813.3

黄玥萌, 郑海英, 杨春艳, 黄加祥, 鄢胜飞, 李舒露, 于农淇, 李孟琪, 尚江华. 摩拉水牛和尼里-拉菲水牛PRKAA2基因SNPs检测及遗传多样性分析[J]. 《中国畜牧兽医》, 2018, 45(5): 1274-1282.

HUANG Yuemeng, ZHENG Haiying, YANG Chunyan, HUANG Jiaxiang, YAN Shengfei, LI Shulu, YU Nongqi, LI Mengqi, SHANG Jianghua. SNPs Detection and Genetic Diversity Analysis of PRKAA2 Gene in Murrah and Nili-Ravi Buffaloes[J]. , 2018, 45(5): 1274-1282.

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