鲁西黄牛MSTN基因上游序列多态性对转录表达的影响

doi:10.16431/j.cnki.1671-7236.2016.07.002

《中国畜牧兽医》---唯一指定的官方网站 ›› 2016, Vol. 43 ›› Issue (7): 1667-1673.doi: 10.16431/j.cnki.1671-7236.2016.07.002

鲁西黄牛MSTN基因上游序列多态性对转录表达的影响

王丹丹¹, 刘晓牧², 张冉¹, 刘桂芬², 万发春², 林浴霜¹

1. 山东大学生命科学学院, 山东省动物细胞与发育生物学重点实验室, 济南 250100;
2. 山东省农业科学院畜牧兽医研究所, 山东省畜禽疫病防治与繁育重点实验室, 济南 250100

收稿日期:2016-01-11 出版日期:2016-07-20 发布日期:2016-07-22
通讯作者: 万发春, 林浴霜 E-mail:wanfc@sina.com;linyushuang@sdu.edu.cn
作者简介:王丹丹(1991-),女,山东曲阜人,硕士生,研究方向:动物发育,E-mail:wangdandan26@126.com刘晓牧(1974-),女,山东阳谷人,副研究员,研究方向:反刍动物营养与饲料科学,E-mail:xmliu2002@163.com;Tel:0531-88967665
基金资助:
山东省农业良种工程重大课题"优质肉牛新品系培育"和肉牛优异品质种质创新利用研究；山东省攻关项目（2014GNC110028）

Effect of MSTN Gene Upstream Sequence Polymorphism on Transcriptional Expression in Luxi Huang Cattle

WANG Dan-dan¹, LIU Xiao-mu², ZHANG Ran¹, LIU Gui-fen², WAN Fa-chun², LIN Yu-shuang¹

1. Provincial Key Laboratory of Animal Cells and Developmental Biology, Life Science College, Shandong University, Jinan 250100, China;
2. Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veternary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China

Received:2016-01-11 Online:2016-07-20 Published:2016-07-22

摘要/Abstract

摘要：

肌肉生长抑制素（myostatin，MSTN）基因是转化生长因子-β（transforming growth factor-β，TGF-β）超家族的新成员。为了研究鲁西黄牛MSTN基因上游序列多态性与转录表达的关系，本试验提取鲁西黄牛腿肌组织细胞的基因组，扩增出MSTN基因上游序列，构建进化树并通过基因测序确定其上游序列中存在单核苷酸多态位点，多态位点位于起始密码子上游805 bp处。构建表达载体，在体外对C2C12细胞进行转染，从而验证MSTN基因上游序列多态位点对转录的影响。结果显示，MSTN基因上游序列中单个核苷酸的改变会影响其下游基因的表达水平。推测在体内MSTN基因上游序列中单核苷酸多态性能够影响基因的转录活性，在调节基因转录的过程中起着重要作用。

关键词: MSTN基因; 上游序列; 单核苷酸多态性; 转录表达

Abstract:

Myostain (MSTN) gene is a member of transforming growth factor-β (TGF-β) superfamily.In order to study the relationship between Luxi Huang cattle MSTN gene upstream sequence polymorphism and the transcriptional expression,DNA was extracted from leg muscle cells of Luxi Huang cattle and then sequenced.After analyzing MSTN upstream sequence, an evolutionary tree was constructed and a SNP site was found at 805 bp of MSTN upstream sequence of initiation codon.Two expression vectors contained the SNP site were constructed to transfect the C2C12 cells in vitro to study the influence of MSTN gene upstream sequence polymorphism on transcription activity.The results showed that single nucleotide change on MSTN gene upstream sequence affected the expression.It suggested that MSTN gene upstream sequence polymorphism of Luxi Huang cattle would affect transcription activity and play an important role in gene expression regulation.

Key words: MSTN gene; upstream sequence; single nucleotide polymorphism; transcriptional expression

中图分类号:

王丹丹, 刘晓牧, 张冉, 刘桂芬, 万发春, 林浴霜. 鲁西黄牛MSTN基因上游序列多态性对转录表达的影响[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2016, 43(7): 1667-1673.

WANG Dan-dan, LIU Xiao-mu, ZHANG Ran, LIU Gui-fen, WAN Fa-chun, LIN Yu-shuang. Effect of MSTN Gene Upstream Sequence Polymorphism on Transcriptional Expression in Luxi Huang Cattle[J]. , 2016, 43(7): 1667-1673.

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鲁西黄牛MSTN基因上游序列多态性对转录表达的影响

Effect of MSTN Gene Upstream Sequence Polymorphism on Transcriptional Expression in Luxi Huang Cattle

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