红眼白水貂FSHβ和NCOA1基因多态性及其与繁殖性状的相关分析

doi:10.16431/j.cnki.1671-7236.2018.02.018

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (2): 429-438.doi: 10.16431/j.cnki.1671-7236.2018.02.018

红眼白水貂FSHβ和NCOA1基因多态性及其与繁殖性状的相关分析

霍自双¹, 刘宗岳², 岳志刚², 杜智恒¹, 丛波², 刘琳玲², 杨福合², 孙余卓³, 白秀娟¹

1. 东北农业大学动物科学技术学院, 哈尔滨 150030;
2. 中国农业科学院特产研究所, 特种经济动物分子生物学国家重点实验室, 农业部特种经济动物遗传育种与繁殖重点实验室, 长春 130112;
3. 鄂尔多斯市农畜产品质量安全中心, 鄂尔多斯 010321

收稿日期:2017-06-21 出版日期:2018-02-20 发布日期:2018-02-10
通讯作者: 白秀娟 E-mail:bxj630306@163.com
作者简介:霍自双(1990-),男,山东菏泽人,硕士生,研究方向:动物生产,E-mail:1510911723@qq.com;刘宗岳(1980-),男,吉林长春人,博士后,研究方向:特种动物育种,E-mail:48740617@qq.com
基金资助:
中国农业科学院创新工程（CAAS-ASTIP-2016-ISAPS）；国家自然科学基金（31702152）

Association Analysis Between Polymorphisms of FSHβ, NCOA1 Genes and Its Reproductive Traits in Pink Eyed White Mink

HUO Zishuang¹, LIU Zongyue², YUE Zhigang², DU Zhiheng¹, CONG Bo², LIU Linling², YANG Fuhe², SUN Yuzhuo³, BAI Xiujuan¹

1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
2. Key Laboratory of Special Economic Animal Genetics and Breeding, Ministry of Agriculture, State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
3. Ordos Quality Safety Center of Agricultural and Livestock Products, Ordos 010321, China

Received:2017-06-21 Online:2018-02-20 Published:2018-02-10

摘要/Abstract

摘要：

本研究旨在检测红眼白水貂促卵泡激素β（follicle-stimulating hormone beta subunit，FSHβ）和核受体辅激活蛋白1（nuclear receptor coactivator 1，NCOA1）基因多态性与繁殖性状的关系。采用单链构象多态性（SSCP）和DNA测序相结合的方法检测了红眼白水貂215个个体的单核苷酸多态性，针对红眼白水貂群体的特点建立合适的统计分析模型，利用SAS 9.4统计软件对候选基因进行多态性分析，并采用最小二乘法分析了总产仔数和产活仔数的遗传效应。结果表明，在红眼白水貂FSHβ基因上存在2个多态位点，分别为内含子1处的g.1228G > A突变和外显子2处的g.1866T > C突变；在NCOA1基因上存在1个多态位点，为第6外显子处g.151536T > C突变。在红眼白水貂群体中，FSHβ基因的优势基因为B等位基因，NCOA1基因的优势基因为A等位基因；FSHβ基因g.1228G > A位点的AA和AB基因型个体在总产仔数、产活仔数上均极显著高于BB基因型（P < 0.01），g.1866T > C位点在总产仔数和产活仔数上呈现BB > AB > AA的趋势；NCOA1基因的AB基因型个体的总产仔数和产活仔数均极显著高于AA基因型（P < 0.01）；g.151536T > C与g.1228G > A的合并基因型对繁殖性状有显著影响（P < 0.05）。因此，可以利用以上突变位点对红眼白水貂的繁殖性状进行标记辅助选择研究。

关键词: 红眼白水貂; 繁殖性状; 单核苷酸多态性; FSHβ 基因; NCOA1基因

Abstract:

This study was performed to investigate the effects of follicle-stimulating hormone beta subunit (FSHβ) and nuclear receptor coactivator 1 (NCOA1) genes on reproductive traits of pink eye White mink. Single nucleotide polymorphisms (SNPs) were detected in 215 individuals by single-strand conformation polymorphism (SSCP)and DNA sequencing, correlation analysis were carried out between candidate gene polymorphisms and reproductive traits using the appropriate model, the effects of different genotypes on reproductive traits, including total number born (TNB) and number born alive (NBA), were analyzed by the least square analysis of SAS 9.4 software. The results showed that two polymorphic sites were found in FSHβ gene, g.1228G > A in intron 1, g.1866 T > C in exon 2. One SNP was found in NCOA1 gene, g.151536T > C in exon 6. B allele of FSHβ gene was predominant allele, A allele of NCOA1 gene was predominant allele; TNB and NBA of AA and AB genotypes in FSHβ g.1228G > A site were extremely significantly higher than BB genotype (P < 0.01); The TNB and NBA for g.1866 > C site genotypes were BB > AB > AA; TNB and NBA of NACOA1 gene AB genotype was extremely significantly higher than AA genotype (P < 0.01); The combing genotypes of 151536T > C-g.1228G > A were significant differences in TNB and NBA (P < 0.05). Therefore, these SNPs could be used for marker assist selection on reproduction traits of the pink eyed White mink.

Key words: pink eyed White mink; reproductive traits; SNP; FSHβ gene; NCOA1 gene

中图分类号:

S813.3

霍自双, 刘宗岳, 岳志刚, 杜智恒, 丛波, 刘琳玲, 杨福合, 孙余卓, 白秀娟. 红眼白水貂FSHβ和NCOA1基因多态性及其与繁殖性状的相关分析[J]. 《中国畜牧兽医》, 2018, 45(2): 429-438.

HUO Zishuang, LIU Zongyue, YUE Zhigang, DU Zhiheng, CONG Bo, LIU Linling, YANG Fuhe, SUN Yuzhuo, BAI Xiujuan. Association Analysis Between Polymorphisms of FSHβ, NCOA1 Genes and Its Reproductive Traits in Pink Eyed White Mink[J]. , 2018, 45(2): 429-438.

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红眼白水貂FSHβ和NCOA1基因多态性及其与繁殖性状的相关分析

Association Analysis Between Polymorphisms of FSHβ, NCOA1 Genes and Its Reproductive Traits in Pink Eyed White Mink

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