大白猪PTGS2基因外显子2多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2018.01.018

摘要/Abstract

摘要：

试验旨在研究前列腺素内过氧化物合酶2（prostaglandin-endoperoxide synthase 2，PTGS2）基因的单核苷酸多态性，并将其与大白猪的繁殖性状进行关联分析，为猪分子遗传标记提供依据。以美系及法系纯种大白猪基因组DNA为模板，构建DNA混合池，通过克隆测序比对，检测猪PTGS2基因的遗传变异；采用PCR-RFLP技术对多态性位点进行基因分型，并与目标性状进行关联分析。结果显示，在猪PTGS2基因外显子2上存在1个g.86A>G碱基突变，并具有Msp Ⅰ酶切位点多态性，且在大白猪群体中检测到AA、AG和GG 3种基因型；哈迪-温伯格平衡检验结果显示，在法系大白猪群体中，PTGS2基因g.86A>G多态性分布达到遗传平衡状态（P>0.05）。关联分析结果表明，在美系大白猪群体中，AA基因型初产母猪弱仔数显著少于AG基因型（P<0.05），AA基因型经产母猪初生窝重显著高于AG基因型（P<0.05）；在法系大白猪群体中，GG基因型个体总产仔数、健仔数和初生窝重均高于AA和AG基因型，但未达到显著水平（P>0.05）。PTGS2基因外显子2 g.86A>G多态性位点显著影响初生窝重和弱仔数，可作为猪分子标记辅助选择育种的潜在位点。

关键词: PTGS2基因; 大白猪; 单核苷酸多态性(SNP); 繁殖性状

Abstract:

This study was aimed to investigate the association between single nucleotide polymorphisms (SNPs) of prostaglandin-endoperoxide synthase 2 (PTGS2) gene exon 2 and the reproductive traits in Large White pigs, which provided a method for the marker-assisted selection. DNA of American and France Large White sows were used to detect the single nucleotide mutation of PTGS2 gene by sequencing alignment, the genotype of polymorphic loci was identified by PCR-RFLP method, the association with reproductive traits in Large White pigs was analyzed. The results showed that a mutation site (g.86A>G) was found of PTGS2 gene exon 2, which changed MspⅠ restriction enzyme cutting site. Three genotypes of AA, AG and GG were detected in Large White pigs. The polymorphism distribution of PTGS2 gene g.86A>G fitted for the Hardy-Weinberg equilibrium in France Large White pigs (P>0.05). In American Large White pigs group, the number of weak piglets in primiparous sow with AA genotype was significantly lower than that of AG genotype (P<0.05), while the litter weight of multiparous sows with AA genotype was significantly higher than that of AG genotype (P<0.05). In France Large White pigs group, the total number born, number of strong piglets and litter weight of GG genotype were higher than those of AA and AG genotypes (P>0.05). The mutation site g.86A>G of PTGS2 gene exon 2 was significant association with the litter weight of multiparous sows and the number of weak piglets,so the polymorphic loci might be one of the candiate molecular markers used in pig breeding.

Key words: PTGS2 gene; Large White pigs; single nucleotide polymorphism (SNP); reproductive traits

中图分类号:

S813.3

聂光伟, 李明杨, 毛翠, 毛慧敏, 王国水, 左波. 大白猪PTGS2基因外显子2多态性及其与繁殖性状的关联分析[J]. 《中国畜牧兽医》, 2018, 45(1): 140-146.

NIE Guangwei, LI Mingyang, MAO Cui, MAO Huimin, WANG Guoshui, ZUO Bo. Polymorphism of PTGS2 Gene Exon 2 and Its Association Analysis with Reproductive Traits in Large White Pigs[J]. , 2018, 45(1): 140-146.

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