天祝白牦牛和甘南牦牛DRB3基因外显子2多态性研究

doi:10.16431/j.cnki.1671-7236.2016.03.027

›› 2016, Vol. 43 ›› Issue (3): 746-753.doi: 10.16431/j.cnki.1671-7236.2016.03.027

天祝白牦牛和甘南牦牛DRB3基因外显子2多态性研究

沙日耐¹, 安天午², 俄广鑫³, 韩建林¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 四川省草原科学研究院, 成都 611731;
3. 西南大学, 重庆 400716

修回日期:2015-12-22 出版日期:2016-03-20 发布日期:2016-03-30
通讯作者: 韩建林 E-mail:h.jianlin@cgiar.org
作者简介:沙日耐(1984-),女,内蒙古呼和浩特人,博士,研究方向:动物遗传育种与繁殖,E-mail:sarinai@163.com
基金资助:
国家"十一五"科技支撑重点项目(2008BADB2B01)

Polymorphism Study on DRB3 Gene Exon 2 in Tianzhu White Yak and Gannan Yak Arlud

SHA Ri-nai¹, AN Tian-wu², E Guang-xin³, HAN Jian-lin¹

1. Institute of Animal Sciences, Chinese Academy of Animal Sciences, Beijing 100193, China;
2. Sichuan Academy of Grassland Science, Chengdu 611731, China;
3. Southwest University, Chongqing 400716, China

Revised:2015-12-22 Online:2016-03-20 Published:2016-03-30

摘要/Abstract

摘要： 试验通过对天祝白牦牛和甘南牦牛DRB3基因外显子2部分CDS序列进行分析,获得其准确单倍型,通过直接测序与聚合酶链式扩增阻碍突变系统(ARMS)扩增法获得干净的单链,对高度杂合的双链进行分型。结果发现65个SNPs,1个密码子的插入缺失,获得30个单倍型,其中有7个单倍型是首次发现,应该是天祝白牦牛和甘南牦牛特有的单倍型。通过对已知牛属所有DRB3基因外显子2单倍型与本研究发现的单倍型进行分析发现天祝白牦牛和甘南牦牛分享普通牛和瘤牛单倍型,可能有普通黄牛和瘤牛的遗传背景,且DRB3基因外显子2的4、25、30、53、59、62、63、66、78氨基酸位点受到了正选择的影响,主要发生在天祝白牦牛群体里,这与天祝白牦牛长期选育白毛有一定的关系。

关键词: 天祝白牦牛; 甘南牦牛; DRB3基因; 外显子2; 多态性

Abstract: In this study,CDS sequence of DRB3 gene exon 2 in Tianzhu White yak and Gannan yak were analyzed,a clean single chain was obtained to get the accurate haplotype by direct sequencing and amplification refractory mutation system (ARMS) method,the double chain of high heterozygosity was typed.The results showed that 65 SNPs and a codon insertion and deletion were discovered,all together separated to 30 haplotypes,in which seven haplotypes were found firstly,it should be peculiar to Tianzhu White yak and Gannan yak.Compared with published bovine DRB3 gene exon 2 haplotypes,the analysis results showed Tianzhu White yak and Gannan yak shared haplotypes of cattle (Bos taurus) and zebu (Bos indicus),which meaned they might crossed with cattle (Bos taurus) and zebu (Bos indicus).PAML analysis showed that 4,25,30,53,59,62,63,66,78 amino acid sites were affected by positive selection,and mainly occurred in Tianzhu yak,it had a certain relation with breeding history of Tianzhu White yak.

Key words: Tianzhu White yak; Gannan yak; DRB3 gene; exon 2; polymorphism

中图分类号:

S813.3

沙日耐, 安天午, 俄广鑫, 韩建林. 天祝白牦牛和甘南牦牛DRB3基因外显子2多态性研究[J]. , 2016, 43(3): 746-753.

SHA Ri-nai, AN Tian-wu, E Guang-xin, HAN Jian-lin. Polymorphism Study on DRB3 Gene Exon 2 in Tianzhu White Yak and Gannan Yak Arlud[J]. , 2016, 43(3): 746-753.

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天祝白牦牛和甘南牦牛DRB3基因外显子2多态性研究

Polymorphism Study on DRB3 Gene Exon 2 in Tianzhu White Yak and Gannan Yak Arlud

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