›› 2015, Vol. 42 ›› Issue (8): 2137-2143.doi: 10.16431/j.cnki.1671-7236.2015.08.031

• 遗传繁育 • 上一篇    下一篇

AQP7基因内含子4多态性与郏县红牛生长性状的关联分析

张婧敏1, 房兴堂2   

  1. 1. 上海农林职业技术学院, 上海 201699;
    2. 江苏师范大学生命科学学院, 徐州 221116
  • 收稿日期:2014-12-19 出版日期:2015-08-20 发布日期:2015-08-27
  • 通讯作者: 房兴堂 E-mail:xtfang@jsnu.edu.cn
  • 作者简介:张婧敏(1986-),女,江苏镇江人,硕士,助教,研究方向:动物生物化学与分子生物学,E-mail:zhangjm@shafc.edu.cn
  • 基金资助:
    上海高校青年教师培养资助项目(ZZnlz12004);江苏省高校科研成果产业化推进工程项目(JHB2012-32);徐州市科技计划项目(XF12C052)

Association Analysis on Polymorphisms of AQP7 Gene Intron 4 with Growth Traits in Jiaxian Cattle

ZHANG Jing-min1, FANG Xing-tang2   

  1. 1. Shanghai Vocational College of Agriculture and Forestry, Shanghai 201699, China;
    2. College of Life Science, Jiangsu Normal University, Xuzhou 221116, China
  • Received:2014-12-19 Online:2015-08-20 Published:2015-08-27

摘要: 本试验旨在研究郏县红牛AQP7基因内含子4的遗传变异,分析其遗传多态性对生长性状的影响。采用PCR-SSCP、DNA序列分析及生物信息学技术探讨郏县红牛AQP7基因内含子4的多态性及其与生长性状的关联情况。结果发现,郏县红牛AQP7基因内含子4存在A、B、C 3个等位基因,其基因频率依次为0.838、0.076、0.086;郏县红牛AQP7基因4种不同基因型与其体重和胸围有显著相关(P< 0.05),与其他指标不存在相关性(P> 0.05),且BC基因型个体的体重和胸围均显著大于其他3种基因型(P< 0.05)。暗示BC基因型是提高郏县红牛生长性状的有利基因型,AQP7基因内含子4的遗传多态性与郏县红牛的生长性状存在相关,因此可作为该品种生长性状分子标记辅助选择的候选基因。

关键词: 郏县红牛; AQP7基因; 多态性; 生长性状; 关联分析

Abstract: The objectives were to research the genetic variations in intron 4 of AQP7 gene in Jiaxian cattle, and analyze the influence of genetic polymorphisms on growth traits.PCR-SSCP technique, DNA sequence analysis and bioinformatics were used to analyze the association between the polymorphisms of the intron 4 of AQP7 gene and growth traits in Jiaxian cattle.The results showed that there were three alleles A, B, C in this locus with the corresponding frequencies of 0.838, 0.076 and 0.086;The polymorphisms of the intron 4 of AQP7 gene had significant association with body weight and chest circumference (P< 0.05), while had no significant association with other growth traits (P> 0.05).The individuals with BC genotype was significantly higher than those of individuals with other genotypes in body weight and chest circumference (P< 0.05).It suggested that BC genotype had positive effect on growth traits, and the correlation was detected between the polymorphisms of the intron 4 of AQP7 gene and growth traits in Jiaxian cattle.So AQP7 gene could be used as a candidate gene for molecular marker assisted selection of growth traits in Jiaxian cattle.

Key words: Jiaxian cattle; AQP7 gene; polymorphisms; growth traits; association analysis

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