《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (6): 1449-1457.doi: 10.16431/j.cnki.1671-7236.2015.06.019

• 动物营养与饲料科学 • 上一篇    下一篇

霉菌毒素吸附剂蒙脱石的耐受性评价

刘少文1, 柏雪1, 程传民2, 郭春华1, 肖英1, 吕娜1   

  1. 1. 西南民族大学生命科学与技术学院, 成都 610041;
    2. 农业部饲料质量监督检验测试中心(成都), 成都 610041
  • 收稿日期:2014-11-27 出版日期:2015-06-20 发布日期:2015-07-23
  • 通讯作者: 柏雪 E-mail:yukivy@163.com
  • 作者简介:刘少文(1990-),男,四川平昌人,硕士,研究方向:动物营养与饲料科学,E-mail:liusw0922@163.com
  • 基金资助:

    农业部专项项目——全国饲料产品中霉菌毒素污染状况摸底调查

Evaluation on Tolerance of Montmorillonite as a Mycotoxin Adsorbent

LIU Shao-wen1, BAI Xue1, CHENG Chuan-min2, GUO Chun-hua1, XIAO Ying1, LV Na1   

  1. 1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;
    2. Feed Products Quality Monitoring Center of the Agricultural Ministry of China (Chengdu), Chengdu 610041, China
  • Received:2014-11-27 Online:2015-06-20 Published:2015-07-23

摘要: 为确定昆明鼠对饲料中霉菌毒素吸附剂蒙脱石的耐受剂量,试验选择60只初始体重为(17±0.12)g的健康雄性昆明系小白鼠,随机分成5个处理组,每组2个重复,每个重复6只.在基础日粮含黄曲霉毒素B1(AFB1)< 10.0 μg/kg的条件下,5个处理组分别添加0(对照组)、0.5%、1%、2%和5%蒙脱石.试验30 d后测定小白鼠生长性能、养分表观消化率、血清生化指标及内脏组织变化.结果表明,添加0.5%蒙脱石显著提高了小白鼠平均日采食量(P< 0.05),添加1%蒙脱石组小白鼠平均日采食量和平均日增重与对照组间均无显著差异(P> 0.05),随着蒙脱石添加水平的升高,小白鼠平均日采食量显著下降(P< 0.05).蒙脱石添加水平为2%时,小白鼠平均日增重与对照组相比无显著差异(P> 0.05),但显著低于0.5%和1%组(P< 0.05).本试验中添加不同水平蒙脱石对小白鼠干物质(DM)、粗蛋白质(CP)和粗脂肪(EE)的表观消化率均未造成显著影响(P> 0.05).随着蒙脱石添加水平的提高,血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及丙二醛(MDA)含量不断升高,除0.5%组与对照组相比差异不显著外(P> 0.05),其他3组均显著高于对照组(P< 0.05).小白鼠组织切片显示,蒙脱石添加水平为5%时,肝脏有不同程度的损伤.综上所述,在本试验条件下,当基础日粮含霉菌毒素在安全范围内时,综合考虑各项指标,昆明系小白鼠对本受试蒙脱石的耐受剂量介于0.5%~2%之间.

关键词: 昆明鼠; 蒙脱石; 生长性能; 血清生化指标; 内脏组织; 耐受性

Abstract: In order to study the tolerance dose of Kunming mice to montmorillonite as a mycotoxin adsorbent in diets,the experiment selected 60 healthy male Kunming mice with initial average body weight of (17±0.12)g,randomly divided them into 5 treatments with two replicates per group and 6 mice per replicate.Five experimental diets were added to 0 (control group),0.5%,1%,2% and 5% montmorillonite,respectively.After 30 days,the growth performance,nutrients apparent digestibility,serum biochemical indices and internal organization were tested to determine the tolerance dose of montmorillonite for Kunming mice.The results showed that the ADFI was significantly increased in 0.5% group (P< 0.05),while there were no significant differences in ADFI and ADG between 1% group and control group (P> 0.05),then the ADFI was significantly decreased with the increasing of montmorillonite supplemental level (P< 0.05).The ADG in 2% group had no significant difference when compared with the control group (P> 0.05),while significantly lower than that in 0.5% and 1% groups (P< 0.05).The apparent digestibility of DM,CP and EE showed no significant effect at any level of montmorillonite (P> 0.05).Serum ALT,AST activities and MDA content were gradually increased with the montmorillonite supplemental level increasing,and that in 1%,2% and 5% groups were significantly higher than that in control group (P< 0.05),but there were no significant differences between 0.5% group and control group (P> 0.05).Tissue sections showed that mice livers were damaged in varying degrees when the montmorillonite supplemental level was 5%.In conclusion,based on a comprehensive consideration of all indexes,it could be seen that the tolerance dose of the tested montmorillonite for Kunming mice was between 0.5% and 2% when mycotoxin contents in diets were in security range.

Key words: Kunming mice; montmorillonite; growth performance; serum biochemical indices; internal organization; tolerance

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