果胶/壳聚糖姜黄素微胶囊制备及其体外人工胃肠道耐受性研究

doi:10.16431/j.cnki.1671-7236.2023.06.015

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (6): 2312-2320.doi: 10.16431/j.cnki.1671-7236.2023.06.015

果胶/壳聚糖姜黄素微胶囊制备及其体外人工胃肠道耐受性研究

徐紫嫣¹, 姜洋¹, 刘学涛¹, 刘柏含¹, 张文¹, 严昌国^1,2,3, 崔莲花^1,2,3

1. 延边大学农学院, 延吉 133002;
2. 东北寒区肉牛科技创新教育部工程研究中心, 延吉 133002;
3. 中国延边黄牛种质资源开发利用学科创新引智基地, 延吉 133002

收稿日期:2022-11-14 出版日期:2023-06-05 发布日期:2023-05-30
通讯作者: 崔莲花 E-mail:cuilianhua@ybu.edu.cn
作者简介:徐紫嫣,E-mail:1003125778@qq.com;姜洋,E-mail:1405207757@qq.com。
基金资助:
吉林省肉牛产业化发展重大科技专项（YDZJ202203CGZH043）；吉林省教育厅重点项目（JJKH20230629KJ）；吉林省大学生创新创业训练项目（S202210184075）；高等学校学科创新引智计划项目（D20034）；延边大学科技规划项目（ydxq202207）

Preparation of Pectin/Chitosan Curcumin Microcapsules and Its Study on Simulated Gastrointestinal Environments

XU Ziyan¹, JIANG Yang¹, LIU Xuetao¹, LIU Bohan¹, ZHANG Wen¹, YAN Changguo^1,2,3, CUI Lianhua^1,2,3

1. Agricultural College of Yanbian University, Yanji 133002, China;
2. Engineering Research Center of North-East Cold Region Beef Cattle Science and Technology Innovation Ministry of Education, Yanji 133002, China;
3. Discipline Innovation and Talent Introduction Base for Development and Utilization of Yellow Cattle Germplasm Resources in Yanbian, Yanji 133002, China

Received:2022-11-14 Online:2023-06-05 Published:2023-05-30

摘要/Abstract

摘要： 【目的】利用微胶囊技术以果胶、壳聚糖为壁材对姜黄素进行包被处理，制备双层包被微胶囊，通过形态特征、机械强度、包埋率等分析确定最佳制备条件，并对其进行体外人工胃肠道耐受性研究。【方法】以8%果胶、不同浓度姜黄素（3.5、4.0、4.5、5.0和5.5 mg/mL）、氯化钙（CaCl₂）溶液（0.3、0.4、0.5和0.6 mol/L）及不同配比姜黄素与果胶溶液（3：7、2：8、1：9（V/V））为第一层包被，筛选果胶姜黄素微胶囊的最佳制备条件；以不同pH （4.2、4.6、5.0、5.4和5.8）的0.8%壳聚糖为第二层包被，筛选制备果胶/壳聚糖姜黄素微胶囊的最佳条件。以筛选的最佳条件制备3组微胶囊（T1、T2和T3），并对其外貌形态、机械强度、包埋率、载药量及体外人工胃肠道耐受性进行评价。【结果】当以8%果胶、4.5 mg/mL姜黄素、姜黄素与果胶配比为1：9（V/V）、0.5 mol/L CaCl₂为第一层包被条件时制得的果胶姜黄素微胶囊包埋率达98%；以pH为4.6、5.0和5.4的0.8%壳聚糖为第二层包被条件时制备的微胶囊大小均匀、形状规则。以筛选的最佳条件制备的3组微胶囊中T1组机械强度、载药量、体外人工胃液中保护率和肠液中释放率均最高。【结论】本研究以果胶和壳聚糖为壁材、姜黄素为芯材，通过筛选的最佳条件制备的果胶/壳聚糖姜黄素微胶囊颗粒形态、机械强度、载药量及体外胃肠道耐受性均较好。研究结果可为开发果胶、壳聚糖为壁材的姜黄素微胶囊的研究提供参考。

关键词: 姜黄素; 微胶囊; 胃肠道耐受性

Abstract: 【Objective】 The double-layer coated microcapsules were prepared using pectin and chitosan as wall materials using microencapsulation technology.The optimal preparation conditions were determined by morphological characteristics, mechanical strength and embedding rate analysis, and the artificial gastrointestinal tolerance of the capsules was studied in vitro.【Method】 The first layer of microcapsule coating was composed of 8% pectin, different concentrations of curcumin (3.5, 4.0, 4.5, 5.0 and 5.5 mg/mL) and calcium chloride (CaCl₂) solution (0.3, 0.4, 0.5 and 0.6 mol/L), and curcumin and pectin solution of different proportions (3:7, 2:8 and 1:9 (V/V)).Screening the best preparation conditions of pectin curcumin microcapsules.Using 0.8% chitosan at different pH (4.2, 4.6, 5.0, 5.4 and 5.8) as the second layer of microcapsule coating, then screened the optimum conditions for preparation of pectin/chitosan curcumin microcapsule.Three groups of microcapsules (T1, T2 and T3) were prepared under the optimal screening conditions, and their appearance, mechanical strength, embedding rate, drug loading and gastrointestinal artificial tolerance in vitro were evaluated.【Result】 The results showed that when 8% pectin, 4.5 mg/mL curcumin, the ratio of curcumin to pectin was 1:9, and 0.5 mol/L CaCl₂ as the first layer of microcapsule coating, the embedding rate of pectin curcumin microcapsules was 98%.When 0.8% chitosan at pH 4.6, 5.0 and 5.4 was used as the second layer of microcapsule coating, the microcapsules showed uniform size and regular shape.Three groups of microcapsules were prepared under the optimal screening conditions, among which the mechanical strength, drug loading, protection rate in artificial gastric fluid and release rate in intestinal fluid in group T1 were the highest.【Conclusion】 In this study, with pectin and chitosan as wall materials and curcumin as core materials, the pectin/chitosan curcumin microcapsules were prepared under the optimal screening conditions, which had good morphology, mechanical strength, drug loading and gastrointestinal tolerance in vitro.The results could provide reference for the development of curcumin microcapsules with pectin and chitosan as wall materials.

Key words: curcumin; microcapsules; gastrointestinal tolerance

中图分类号:

S816.79

徐紫嫣, 姜洋, 刘学涛, 刘柏含, 张文, 严昌国, 崔莲花. 果胶/壳聚糖姜黄素微胶囊制备及其体外人工胃肠道耐受性研究[J]. 中国畜牧兽医, 2023, 50(6): 2312-2320.

XU Ziyan, JIANG Yang, LIU Xuetao, LIU Bohan, ZHANG Wen, YAN Changguo, CUI Lianhua. Preparation of Pectin/Chitosan Curcumin Microcapsules and Its Study on Simulated Gastrointestinal Environments[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2312-2320.

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果胶/壳聚糖姜黄素微胶囊制备及其体外人工胃肠道耐受性研究

Preparation of Pectin/Chitosan Curcumin Microcapsules and Its Study on Simulated Gastrointestinal Environments

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