果胶/壳聚糖合生元微胶囊制备及其体外人工胃肠道耐受性和储藏性能研究

doi:10.16431/j.cnki.1671-7236.2020.09.012

摘要/Abstract

摘要： 本研究以果胶、壳聚糖为壁材，乳酸片球菌和菊粉为壁芯，运用挤压法制备果胶/壳聚糖合生元微胶囊（PC（PA-I）MCs），并以包埋率、颗粒形态、机械强度、体外胃肠道耐受性及贮藏性能等为考核指标，研究合生元微胶囊包被工艺。试验分为3组，分别为游离状态的乳酸片球菌组（PA）、单独包埋乳酸片球菌制得的微胶囊组（PC（PA）MCs），菊粉与乳酸片球菌一同包埋制得的合生元微胶囊组（PC（PA-I）MCs）。结果表明，当果胶的质量浓度为5 g/100 mL、菊粉添加量为25 mg/mL、壳聚糖质量浓度为0.8 g/100 mL时，PC（PA-I）MCs组的微胶囊外观、粒径、机械强度和包埋率较好；通过体外人工胃肠道耐受性试验发现，PC（PA-I）MCs组在体外人工胃液中比较稳定（P<0.05），在体外人工肠液中30 min开始迅速释放，到2 h后微胶囊基本完全崩解。通过4周的储藏试验比较发现，PA组乳酸片球菌在冷藏和常温下，菌体存活率下降明显，而添加菊粉的PC（PA-I）MCs组菌体存活率下降幅度较小，且PC（PA-I）MCs组的菌体存活率高于PC（PA）MCs组（P>0.05）；室温条件下PC（PA-I）MCs组的菌体存活率高于PC（PA）MCs组（P>0.05）。综上所述，以菊粉为益生元的果胶/壳聚糖微胶囊对乳酸片球菌有较好的包埋效果。

关键词: 乳酸片球菌; 微胶囊; 胃肠道耐受性; 储藏性能

Abstract: Pediococcus acidilactici and inulin were used as wall cores,with pectin and chitosan as the shell material.The conditions of microencapsulation were optimized by analyzing the embedding rate,particle morphology,the mechanical strength,gastrointestinal tolerance in vitro and storage performance.The experimental group was divided into three groups:Free Pediococcus acidilactici group (PA),microcapsule group made by embedding Pediococcus acidilactici alone (PC(PA)MCs),synbiotic microcapsule group made by embedding inulin and Pediococcus acidilactici (PC(PA-I)MCs).The results showed that the appearance,particle size,mechanical strength and embedding rate of microcapsules were better when the mass concentration of pectin was 5 g/100 mL,the content of inulin was 25 mg/mL and the mass concentration of chitosan was 0.8 g/100 mL.Through the gastrointestinal tolerance test in vitro, it was found that PC(PA-I)MCs group was relatively stable in the artificial gastric juice in vitro (P<0.05).At the same time,it began to release rapidly in the artificial intestinal fluid in vitro at 30 min and it was almost completely disintegrated after 2 h.It was found that the cell survival rate of PA group decreased significantly at cold and room temperature through the storage test for 4 weeks.While the cell survival rate of PC(PA-I)MCs group decreased slightly.And the cell survival rate of PC(PA-I)MCs group was higher than that of PC(PA)MCs group (P>0.05).At room temperature,the cell survival rate of PC(PA-I)MCs group was higher than that of PC(PA)MCs group (P>0.05).In summary,pectin/chitosan microcapsules with inulin as probiotics had a better embedding effect on Pediococcus acidilactici.

Key words: Pediococcus acidilacticis; microcapsules; gastrointestinal tolerance; storage stability

中图分类号:

S816.79

崔乃元, 高青山, 严昌国, 崔莲花. 果胶/壳聚糖合生元微胶囊制备及其体外人工胃肠道耐受性和储藏性能研究[J]. 中国畜牧兽医, 2020, 47(9): 2799-2807.

CUI Naiyuan, GAO Qingshan, YAN Changguo, CUI Lianhua. Preparation of Pectin/Chitosan Synbiotic Microcapsules and Its Study on Simulated Gastrointestinal Environments and Storage Stability[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2799-2807.

参考文献

[1] LI X Y,CHEN X G,SUN Z W,et al.Preparation of alginate/chitosan/carboxymethyl chitosan complex microcapsules and application in Lactobacillus casei ATCC 393[J].Carbohydrate Polymers,2011,83(4):1479-1485.
[2] VALENTE J F A,GASPAR V M,ANTUNES B P,et al.Microencapsulated chitosan-dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration[J].Polymer,2013,54(1):5-15.
[3] GÜNTER E A,POPEYKO O V.Calcium pectinate gel beads obtained from callus cultures pectins as promising systems for colon-targeted drug delivery[J].Carbohydrate Polymers,2016,147:490-499.
[4] DUAN H G,LÜ S Y,GAO C M,et al.Mucoadhesive microparticulates based on polysaccharide for target dual drug delivery of 5-aminosalicylic acid and curcumin to inflamed colon[J].Colloids and Surfaces B:Biointerfaces,2016,145:510-519.
[5] PRUDHVIRAJ G,VAIDYA Y,SINGH S K,et al.Effect of co-administration of probiotics with polysaccharide based colon targeted delivery systems to optimize site specific drug release[J].European Journal of Pharmaceutics and Biopharmaceutics,2015,97:164-172.
[6] 魏轶男,黄倩倩,吕亚军,等.菊粉对肉仔鸡生长性能、免疫器官指数及抗氧化指标的影响[J].西北农林科技大学学报(自然科学版),2013,41(11):13-18. WEI Y N,HUANG Q Q,LYU Y J,et al.Effects of inulin on growth performance,immune organs indexes and antioxidantion of broliers[J].Journal of Northwest A&F University(Natural Science Edition),2013,41(11):13-18.(in Chinese)
[7] 李海英,张俐华,翟卫爽,等.菊粉替代饲用抗生素对白羽肉鸡生产性能和免疫器官指数的影响[J].新疆农业科学,2014,51(3):558-563. LI H Y,ZHANG L H,ZHAI W S,et al.Effects of inulin used as substitute for antibiotics feed additive on production performance and immune organ index of white broilers[J].Xinjiang Agricultural Sciences,2014,51(3):558-563.(in Chinese)
[8] 管倩侽.饲粮添加菊糖对肉仔鸡生产性能、消化代谢及非特异性免疫功能的影响[D].沈阳:沈阳农业大学,2017. GUAN Q N.Effects of dietary inulin supplementation on growth performance,digestive metabolism and non-specific immune function of broilers[D].Shenyang:Shenyang Agricultural University,2017.(in Chinese)
[9] SASAKI H,MIYAKAWA H,WATANABE A,et al.Mice microbiota composition changes by inulin feeding with a long fasting period under a Two-Meals-Per-Day schedule[J].Nutrients,2019,11:2802.
[10] AKRAM W,GARUD N,JOSHI R.Role of inulin as prebiotics on inflammatory bowel disease[J].Drug Discoveries & Therapeutics,2019,13(1):1-8.
[11] DELZENNE N M,DAUBIOUL C,NEVRINCK A,et al.Inulin and oligofructose modulate lipid metabolism in animals:Review of biochemical events and future prospects[J].British Journal of Nutrition,2002,87:S255-S259.
[12] 张瑞.果胶+明胶/乳清蛋白双层微胶囊包埋鼠李糖乳杆菌研究[D].哈尔滨:东北农业大学,2017. ZHANG R.Study on Lactobacillus rhamnosus treated by pectin+gelatin/whey protein double layer micro-capsules[D].Harbin:Northeast Agricultural University,2017.(in Chinese)
[13] 方一,李路海,胡朝丽,等.微胶囊机械性能表征方法的研究[J].北京印刷学院学报,2008,16(6):34-37. FANG Y,LI L H,HU Z L,et al.Characterization method research of the mechanical properties of microcapsules[J].Journal of Bejing Institute of Graphic Communication,2018,16(6):34-37.(in Chinese)
[14] JIANG T,LI H S,HAN G G,et al.Oral delivery of probiotics in poultry using pH-sensitive tablets[J].Journal of Microbiology and Biotechnology,2017,27(4):739-746.
[15] 杨飞,周华,夏书芹,等.壳聚糖-果胶钙微球的制备及其体外溶胀释放性的研究[J].食品工业科技,2013,34,(12):137-140. YANG F,ZHOU H,XIA S Q,et al.Study on chitosan-calcium pectin microsphere preparation and in vitro swelling and release properties[J].Science and Technology of Food Industry,2013,34(12):137-140.(in Chinese)
[16] 周剑忠,董明盛,江汉湖.乳酸菌细胞的液芯包嚢固定化技术及应用[J].食品与发酵工业,2004,30(2):82-86. ZHOU J Z,DONG M S,JIANG H H.Application of liquid-core encapsulation of immobolied cell for LAB[J].Food and Fermentation Industries,2004,30(2):82-86.(in Chinese)
[17] PAULRAJ K,SATISH K R,YUVARAJ N,et al.Effect of cryopreservation and microencapsulation of lactic acid bacterium Enterococcus faecium MC13 for long-term storage[J].Biochemical Engineering Journal,2011,58-59:140-147.
[18] 吕利军,菊粉制备和乳酸菌微胶囊化包被的研究[D].呼和浩特:内蒙古农业大学,2008. LYU L J.Preparation of inulin and study on Lactobacillus microencapsulation[D].Huhhot:Inner Mongolia Agricultural University,2008.(in Chinese)
[19] KHURANA R,KULDEEP S,BHARTI S,et al.Tamarindus indica pectin blend film composition for coating tablets with enhanced adhesive force strength[J].Carbohydrate Polymers,2014,102:55-65.
[20] ZAEIM D,GHORANI B,SARABIJAMAB M,et al.Electrospray assisted fabrication of hydrogel microcapsules by single- and double-stage procedures for encapsulation of probiotics[J].Food & Bioproducts Processing,2017,102:250-259.
[21] 董琦,徐威,张碟,等.负载硒蛋白果胶微凝胶的制备、结构表征及体外消化特性研究[J].食品工业科技,2020,41(8):37-42. DONG Q,XU W,ZHANG D,et al.Preparation,structure characterization and in vitro digestibility of selenoprotein rich pectin microgel[J].Science and Technology of Food Industry,2020,41(8):37-42.(in Chinese)
[22] CUI L H,YAN C G,LI H S,et al.A new method of producing a natural antibacterial peptide by encapsulated probiotics internalized with inulin nanoparticles as prebiotics[J].Journal of Microbiol Biotechnolgy,2018,28(4):510-519.
[23] 张国芳,王婷婷,刘丽波,等.内源乳化法制备干酪乳杆菌微胶囊[J].中国乳品工业,2017,45(3):15-20. ZHANG G F,WANG T T,LIU L B,et al.Internal emulsification microcapsule preparation of Lactobaci-lluscasei[J].China Dairy Industry,2017,45(3):15-20.(in Chinese)
[24] 李伟,纪鹃,陈晓红,等.海藻酸钠/壳聚糖双层合生元微胶囊制备及储藏稳定性和控制性释放[J].乳业科学与技术,2013,36(1):8-12. LI W,JI J,CHEN X H,et al.Preparation of sodium alginate/chitosan symbiotic microcapsules and its storage stability and controlled release[J].Journal of Dairy Science and Technology,2013,36(1):8-12.(in Chinese)
[25] ORTAKCI F,BROADBENT J R,MCMAHON W R.Survival of microencapsulated probiotic Lactobacillus paracasei LBC-1e during manufacture of mozzarella cheese and simulated gastric digestion[J].Journal of Dairy Science,2012,95(11):6274-6281.