暗斑对蛋鸡蛋品质的影响及其与肠道功能的关系

doi:10.16431/j.cnki.1671-7236.2023.06.014

摘要/Abstract

摘要： 【目的】探究暗斑对蛋鸡蛋品质的影响以及暗斑蛋形成与蛋鸡肠道功能的关系。【方法】选取100只276日龄、饲养环境相同的济宁百日鸡母鸡，每天09：00收集鸡蛋，统计每只母鸡产暗斑蛋情况，试验期42 d。选择10只连续产1级暗斑蛋率高的蛋鸡为对照组，10只连续产4级暗斑蛋率高的蛋鸡为暗斑组，筛选暗斑蛋与非暗斑蛋各60枚，测定蛋品质；试验于42 d屠宰蛋鸡，采集空肠和回肠组织，观察肠道组织形态，测定空肠与回肠组织消化酶、ATP酶活性和抗氧化因子水平。【结果】与对照组相比，暗斑组的蛋壳厚度、蛋壳比例极显著升高（P<0.01），蛋黄颜色显著降低（P<0.05）；暗斑组蛋鸡空肠脂肪酶、钠钾ATP酶、谷胱甘肽过氧化物酶活性和总抗氧化能力显著或极显著降低（P<0.05；P<0.01），回肠淀粉酶、钙镁ATP酶活性显著降低（P<0.05）；空肠和回肠绒毛高度、隐窝深度、绒隐比差异不显著（P>0.05）。【结论】鸡蛋暗斑影响蛋品质，导致蛋壳厚度、蛋壳比例增加，蛋黄颜色降低；产暗斑蛋鸡的肠道部分消化酶及抗氧化能力降低，推测暗斑蛋的产生与肠道消化吸收、抗氧化能力存在关联。

关键词: 济宁百日鸡; 暗斑; 蛋品质; 肠道

Abstract: 【Objective】 The purpose of this experiment was to explore the effect of translucent on egg quality and the relationship between the formation of translucent eggs and the intestinal function of laying hens.【Method】 A total of 100 Jining Bairi chickens aged 276 days with the same feeding environment were selected.Eggs were collected at 09:00 every day and translucent production of each hen was counted.The experiment lasted for 42 days.Ten laying hens with high translucent rate of grade 1 were selected as the control group, and ten laying hens with high translucent rate of grade 4 were selected as the translucent group.60 translucent eggs and 60 normal eggs were selected to determine the egg quality.Jejunum and ileum tissues were collected from laying hens on day 42.The morphology of intestinal tissues was observed, and the activities of digestive enzymes, ATPase and antioxidant factors in jejunum and ileum tissues were determined.【Result】 The results showed that, compared with the control group, the eggshell thickness and eggshell proportion in translucent group were extremely significantly increased (P<0.01), while the egg yolk color was significantly decreased (P<0.05).The activities of lipase, sodium-potassium ATPase, glutathione peroxidase and total antioxidant capacity of jejunum of laying hens in translucent group were significantly or extremely significantly decreased (P<0.05 or P<0.01).The activities of amylase and calcium and magnesium ATPase of ileal in translucent group were significantly decreased (P<0.05).There were no significant differences in the villus height, crypt depth and ratio of villus to crypt of jejunum and ileum between translucent and control group (P>0.05).【Conclusion】 Eggshell translucent affected egg quality, resulting in eggshell thickness, eggshell ratio increased and yolk color decreased.Some intestinal digestive enzymes and antioxidant capacity of laying hens with eggshell translucent were decreased, so it was speculated that the production of translucent eggs was related to intestinal digestion, absorption and antioxidant capacity.

Key words: Jining Bairi chicken; translucent; egg quality; intestinal

中图分类号:

S831

杜元军, 段国超, 刘玮, 李大鹏, 韩海霞, 雷秋霞, 周艳, 刘杰, 王杰, 曹顶国, 李福伟, 陈甫. 暗斑对蛋鸡蛋品质的影响及其与肠道功能的关系[J]. 中国畜牧兽医, 2023, 50(6): 2305-2311.

DU Yuanjun, DUAN Guochao, LIU Wei, LI Dapeng, HAN Haixia, LEI Qiuxia, ZHOU Yan, LIU Jie, WANG Jie, CAO Dingguo, LI Fuwei, CHEN Fu. Effect of Translucent on Egg Quality and Its Relationship with Intestinal Function of Laying Hen[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2305-2311.

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