霉菌毒素吸附剂蒙脱石的耐受性评价

doi:10.16431/j.cnki.1671-7236.2015.06.019

摘要/Abstract

摘要： 为确定昆明鼠对饲料中霉菌毒素吸附剂蒙脱石的耐受剂量,试验选择60只初始体重为(17±0.12)g的健康雄性昆明系小白鼠,随机分成5个处理组,每组2个重复,每个重复6只.在基础日粮含黄曲霉毒素B₁(AFB₁)< 10.0 μg/kg的条件下,5个处理组分别添加0(对照组)、0.5%、1%、2%和5%蒙脱石.试验30 d后测定小白鼠生长性能、养分表观消化率、血清生化指标及内脏组织变化.结果表明,添加0.5%蒙脱石显著提高了小白鼠平均日采食量(P< 0.05),添加1%蒙脱石组小白鼠平均日采食量和平均日增重与对照组间均无显著差异(P> 0.05),随着蒙脱石添加水平的升高,小白鼠平均日采食量显著下降(P< 0.05).蒙脱石添加水平为2%时,小白鼠平均日增重与对照组相比无显著差异(P> 0.05),但显著低于0.5%和1%组(P< 0.05).本试验中添加不同水平蒙脱石对小白鼠干物质(DM)、粗蛋白质(CP)和粗脂肪(EE)的表观消化率均未造成显著影响(P> 0.05).随着蒙脱石添加水平的提高,血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及丙二醛(MDA)含量不断升高,除0.5%组与对照组相比差异不显著外(P> 0.05),其他3组均显著高于对照组(P< 0.05).小白鼠组织切片显示,蒙脱石添加水平为5%时,肝脏有不同程度的损伤.综上所述,在本试验条件下,当基础日粮含霉菌毒素在安全范围内时,综合考虑各项指标,昆明系小白鼠对本受试蒙脱石的耐受剂量介于0.5%~2%之间.

关键词: 昆明鼠; 蒙脱石; 生长性能; 血清生化指标; 内脏组织; 耐受性

Abstract: In order to study the tolerance dose of Kunming mice to montmorillonite as a mycotoxin adsorbent in diets,the experiment selected 60 healthy male Kunming mice with initial average body weight of (17±0.12)g,randomly divided them into 5 treatments with two replicates per group and 6 mice per replicate.Five experimental diets were added to 0 (control group),0.5%,1%,2% and 5% montmorillonite,respectively.After 30 days,the growth performance,nutrients apparent digestibility,serum biochemical indices and internal organization were tested to determine the tolerance dose of montmorillonite for Kunming mice.The results showed that the ADFI was significantly increased in 0.5% group (P< 0.05),while there were no significant differences in ADFI and ADG between 1% group and control group (P> 0.05),then the ADFI was significantly decreased with the increasing of montmorillonite supplemental level (P< 0.05).The ADG in 2% group had no significant difference when compared with the control group (P> 0.05),while significantly lower than that in 0.5% and 1% groups (P< 0.05).The apparent digestibility of DM,CP and EE showed no significant effect at any level of montmorillonite (P> 0.05).Serum ALT,AST activities and MDA content were gradually increased with the montmorillonite supplemental level increasing,and that in 1%,2% and 5% groups were significantly higher than that in control group (P< 0.05),but there were no significant differences between 0.5% group and control group (P> 0.05).Tissue sections showed that mice livers were damaged in varying degrees when the montmorillonite supplemental level was 5%.In conclusion,based on a comprehensive consideration of all indexes,it could be seen that the tolerance dose of the tested montmorillonite for Kunming mice was between 0.5% and 2% when mycotoxin contents in diets were in security range.

Key words: Kunming mice; montmorillonite; growth performance; serum biochemical indices; internal organization; tolerance

中图分类号:

S816.7

刘少文, 柏雪, 程传民, 郭春华, 肖英, 吕娜. 霉菌毒素吸附剂蒙脱石的耐受性评价[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(6): 1449-1457.

LIU Shao-wen, BAI Xue, CHENG Chuan-min, GUO Chun-hua, XIAO Ying, LV Na. Evaluation on Tolerance of Montmorillonite as a Mycotoxin Adsorbent[J]. , 2015, 42(6): 1449-1457.

参考文献

[1] 王若军,苗朝华,张振雄.中国饲料及饲料原料受霉菌毒素污染的调查报告[J].饲料工业,2003,24(7):53-54.

[2] Çelik K,Uzatící A,Co?kun B,et al.Current developments in removal of mycotoxins by biological methods and chemical adsorbents[J].Journal of Hygienic Engineering and Design,2013,3:17-20.

[3] 周闯,何成华,司慧民,等.2012年国内饲料及原料霉菌毒素污染调查分析[J].畜牧与兽医,2014,46(1):81-84.

[4] 高潮,郑云峰,王祖平,等.饲料中霉菌毒素脱毒剂的发展趋势[J].饲料研究,2014,5:7-9.

[5] 熊江林,刘建新.日粮黄曲霉毒素B₁向牛奶的转化及其对奶牛生产性能与健康的影响[D].杭州:浙江大学,2014.

[6] Takagi M,Uno S,Kokushi E,et al.Measurement of urinary zearalenone concentrations for monitoring natural feed contamination in cattle herds:On-farm trials[J].Journal of Animal Science,2011,89(1):287-296.

[7] Miazzo R,Peralta M F,Magnoli C,et al.Efficacy of sodium bentonite as a detoxifier of broiler feed contaminated with aflatoxin and fumonisin[J].Poultry Science,2005,84(1):1-8.

[8] 姜淑贞,杨维仁,杨在宾,等.玉米赤霉烯酮饲粮添加蒙脱石对断奶仔猪脾脏和外周血免疫指标的影响[J].中国粮油学报,2013,28(7):60-64.

[9] 胡屹屹,苏永腾,何孔旺.新型蒙脱石对仔猪血液生化指标的影响[J].江西农业学报,2014,26(4):90-93.

[10] 刘国栋,薛敏,李军国,等.杂交鳃对饲料中霉菌毒素吸附剂蒙脱石的耐受性评价[J].动物营养学报,2013,25(8):1886-1895.

[11] 王黎文,丁健,张建刚,等.霉菌毒素吸附剂蒙脱石对泌乳奶牛生产性能和血清生化指标的影响[J].动物营养学报,2013,25(7):1595-1602.

[12] 张俊平,李洁云.蒙脱石对不同日龄仔猪生长性能和腹泻的影响[J].中国饲料,2014,6:15-16.

[13] 雷春龙.高精料日粮中添加不同吸附剂对肉牛体内内毒素吸附、养分消化率、生产性能及选择性采食的影响[D].重庆:西南大学,2013.

[14] 杨荣芳,郝生宏,王敏奇,等.纳米载铜蒙脱石对生长肥育猪生产性能及血液生化指标的影响[J].中国畜牧兽医,2010,37(2):40-43.

[15] 张建刚,赵国琦,林淼,等.日粮霉菌毒素吸附剂添加水平对奶牛营养物质表观消化率和血液指标的影响[J].中国畜牧杂志,2012,48(23):44-48.

[16] 胡彩虹,徐勇,熊莉,等.载铜蒙脱石对尼罗罗非鱼肠道菌群和消化机能的影响[J].水生生物学报,2008,32(6):881-887.

[17] Che Z Q,Liu Y L,Wang H R,et al.The protective effects of different mycotoxin adsorbents against blood and liver pathological changes induced by mold-contaminated feed in broilers[J].Asian-Aust J Anim Sci,2011,24:250-257.

[18] 王聪,周凌云,董淑慧.蒙脱石主要作用机理及其在反刍动物生产中的应用研究进展[J].中国畜牧兽医,2012,39(8):108-110.

[19] Davidson J N,Babish J G,Delaney K A.Hydrated sodium calcium aluminosilicate decrease the bioavailabilitv of aflatoxin in the chicken[J].Poult Sci,1987,58:66-89.

[20] 吴小兰,刘先洲.脂肪肝与谷丙转氨酶、血糖及血脂的相关性分析[J].公共卫生与预防医学,2007,4:10-12.

[21] 崔艳华,张兰威.谷氨酰胺转氨酶研究进展[J].生物技术通报,2009,1:31-36.

[22] Kubena L F,Harvey R B,Huff W E,et al.Efficacy of a hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin and diacetoxyscirpenol[J].Poultry Science,1993,72(1):51-59.

[23] 赵珩伊,余冰,毛湘冰,等.水合硅铝酸钠钙对生长肥育猪生长性能、养分表观消化率及抗氧化能力的影响[J].动物营养学报,2013,25(3):571-578.

[24] 吴秋珏,吴亚男,田金可,等.斜发沸石对肉鸡肠道抗氧化功能、脂肪沉积与肉品质的影响[J].畜牧兽医学报,2013,44(1):57-65.

[25] Tanu C G,Liu J P,Wen W Y.Effects of zinc-deficient ration on biochemical and physiological indices and Zn,Cu content of tissues in pigs[J].Chinese Journal of Veterinary Science,1998,27(1):49-55.

[26] 韩怀动,张军民.饲料添加剂——膨润土研究进展[J].饲料工业,2003,24(9):28-31.

[27] 林洁,梁学武.膨润土对大鼠营养生理作用机理及安全性评价[D].福州:福建农林大学,2010.

[28] 李海宁,王峻,何万领.蒙脱石的药用价值及存在问题[J].湖北农业科学,2003,2:93-94.