亚洲黑熊主要组织相容性复合体DQA及DRB基因外显子2多态性分析

›› 2014, Vol. 41 ›› Issue (10): 157-164.

亚洲黑熊主要组织相容性复合体DQA及DRB基因外显子2多态性分析

高文杰^1,2, 朱自严²

1. 华东师范大学, 上海 200241;
2. 上海市血液中心, 上海 200051

收稿日期:2014-03-10 出版日期:2014-10-20 发布日期:2014-10-29
通讯作者: 朱自严 E-mail:zhuziyan@sbc.org.cn
作者简介:高文杰(1988－),男,山东人,硕士,研究方向:血液免疫学和动物血型。
基金资助:
卫生行业科研专项(201002005)

Polymorphic Analysis of MHC-DQA and MHC-DRB Gene Exon 2 in Asiatic Black Bear(Ursus thibetanus)

GAO Wen-jie^1,2, ZHU Zi-yan²

1. East China Normal University, Shanghai 200241, China;
2. Shanghai Blood Center, Shanghai 200051, China

Received:2014-03-10 Online:2014-10-20 Published:2014-10-29

摘要/Abstract

摘要： 试验旨在分析亚洲黑熊主要组织相容性复合体(major histocompatibility complex,MHC) DQA和DRB基因外显子2多态性。采用PCR扩增和克隆测序等方法对来自云南地区的40只亚洲黑熊DNA样本进行研究。结果发现,在40只亚洲黑熊样本中检测出了9个DQA外显子2等位基因和13个DRB外显子2等位基因,和大熊猫相比,亚洲黑熊在DQA和DRB基因外显子2位点上有较高的多态性。通过对推导出的氨基酸序列抗原结合位点的同义替换和非同义替换的比较,证明了DQA和DRB位点的平衡选择作用。

关键词: 亚洲黑熊; DQA基因; DRB基因; 多态性; 平衡选择

Abstract: In this study,we analyze the allelic diversity of the DQA and DRB gene exon 2 locus on major histocompatibility complex (MHC) gene of Asiatic Black bear. Researced by cloning,sequencing and statistical analysis in the 40 Asiatic Black bear (Ursus thibetanus) from Yunnan province. The results showed that nine alleles of the DQA gene exon 2 and thirteen alleles of the DRB gene exon 2 were identified from 40 individuals. Relative to the giant panda,the diversity of DQA and DRB locus in Asian Black bears were more polymorphic. Comparisons of non-synonymous and synonymous substitutions in the antigen binding sites of deduced amino acid sequences indicated evidence for balancing selection on DRB and DQA locus.

Key words: Ursus thibetanus; DQA gene; DRB gene; polymorphic; balancing selection

中图分类号:

S813.3

高文杰, 朱自严. 亚洲黑熊主要组织相容性复合体DQA及DRB基因外显子2多态性分析[J]. , 2014, 41(10): 157-164.

GAO Wen-jie, ZHU Zi-yan. Polymorphic Analysis of MHC-DQA and MHC-DRB Gene Exon 2 in Asiatic Black Bear(Ursus thibetanus)[J]. , 2014, 41(10): 157-164.

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