DNA疫苗的研究进展

›› 2013, Vol. 40 ›› Issue (1): 72-76.

DNA疫苗的研究进展

杨海, 王芳宇

衡阳师范学院生命科学系, 湖南衡阳 421008

收稿日期:2012-06-20 出版日期:2013-01-20 发布日期:2013-01-14
作者简介:杨海(1978-),男,湖南人,讲师,博士,从事畜牧兽医的教学与科研工作。
基金资助:
湖南省自然科学基金项目(10JJ6052);衡阳市科技计划项目(2012KN24);衡阳师范学院产学研用项目(12CXYY07)。

Research Progress of DNA Vaccine

YANG Hai, WANG Fang-yu

Department of Life Science, Hengyang Normal University, Hengyang 421008, China

Received:2012-06-20 Online:2013-01-20 Published:2013-01-14

摘要/Abstract

摘要： DNA疫苗是在分子生物学技术基础上发展起来的第3代新型疫苗,已体现出其竞争优势和应用潜能。同传统的疫苗相比,DNA疫苗具有免疫效果好、生产成本低、临床应用方便等优点,但同样存在安全性的担忧。文章回顾了DNA疫苗的发展简史,阐述了DNA疫苗的免疫机理,分析了DNA疫苗研究现状,并对DNA疫苗的安全性提出了自己的观点与看法,旨在为DNA疫苗研究提供参考。

关键词: DNA疫苗; 免疫机理; 安全性; 研究进展

Abstract: DNA vaccine was the third generation of vaccine, and had reflected the competitive advantages and application potential in the past. DNA vaccine was developed on the basis of molecular biology technologies.Compared with traditional vaccines,it had more advantages,such as good immune effect, low production cost, and convenient for the clinical application, but it also could be found safety concerns. To provide the references for DNA vaccine researchers,the DNA vaccine history,immune mechanism and research status quo were summarized, and the viewpoint about its security was presented in present paper.

Key words: DNA vaccine; immune mechanism; security; research progress

中图分类号:

S852.4

杨海, 王芳宇. DNA疫苗的研究进展[J]. , 2013, 40(1): 72-76.

YANG Hai, WANG Fang-yu. Research Progress of DNA Vaccine[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(1): 72-76.

参考文献

1 王昌青,杨兵. DNA疫苗研究进展[J].中国畜牧兽医,2009,36(12):143~146.
2 张亮,阎瑾琦,王越,等. 可复制型抗肿瘤DNA疫苗PSCK-2PFcGB的构建及体内外表达[J].南方医科大学学报,2011,31(6):937~942.
3 李娜,孙元,仇华吉. 猪瘟DNA疫苗研究进展[J].生物工程学报,2010,26(3):281~289.
4 杜宇,樊明文,李宇红,等. 防龋DNA疫苗pGJA-P/VAX免疫小鼠后的抗DNA抗体检测[J].口腔医学研究,2006,22(5):465~467.
5 步志高,赵晓岩,刘长军,等. DNA免疫诱导SPF鸡对传染性法氏囊病的免疫保护反应[J].中国预防兽医学报,2000,22(5):325~328.
6 梁雪芽,方维焕,江玲丽. 鸡新城疫口服DNA疫苗的安全性、稳定性与免疫效力[J].生物工程学报,2003,19(1):24~29.
7 Barber G N.Cytoplasmic DNA innate immune pathways[J]. Immunol Rev,2011,243(1):99~108.
8 Carvalho J A, Prazeres D M, Monteiro G A.Bringing DNA vaccines closer to commercial use[J]. IDrugs, 2009,12(10):642~647.
9 Coban C, Kobiyama K, Aoshi T, et al. Novel strategies to improve DNA vaccine immunogenicity[J]. Curr Gene Ther, 2011, 11(6):479~484.
10 Cristillo A D, Weiss D, Hudacik L, et al. Persistent antibody and T cell responses induced by HIV-1 DNA vaccine delivered by electroporation[J]. Biochem Biophys Res Commun, 2008,366(1):29~35.
11 Ferraro B, Morrow M P, Hutnick N A, et al. Clinical applications of DNA vaccines: Current progress[J]. Clin Infect Dis, 2011,53(3):296~302.
12 Fowler V L, Barnett P V.Progress in the development of DNA vaccines against foot-and-mouth disease[J]. Expert Rev Vaccines, 2012,11(4):481~493.
13 Fyana E F, Rohinson H L, Wehsler R G, et al. Use of DNA encoding influenza hemagglulinin as avian influenza vaccine[J].DNA Cell Biol, 1993,12(9):785~789.
14 Germain R N. MHC-dependent antigen processing and peptide presentation: Providining ligands for T lymphocyte activation[J].Cell, 1994,76:287~299.
15 Iurescia S, Fioretti D, Fazio V M, et al. Design and pre-clinical development of epitope-based DNA vaccines against B-cell iymphoma[J]. Curr Gene Ther, 2011,11(5):414~422.
16 Jazayeri S D, Ideris A, Zakaria Z, et al. Attenuated Salmonella typhimurium SV4089 as a potential carrier of oral DNA vaccine in chickens[J]. J Biomed Biotechnol, 2012,264986.
17 Rohinson H L, Hunl L A, Wehsler R G, et al. Protection against a lethal influenza virus challenge by immunization with a haemagglulin in expressing plasmid DNA[J].Vaccine, 1993,11(9):957~969.
18 Saade F, Petrovsky N.Technologies for enhanced efficacy of DNA vaccines[J]. Expert Rev Vaccines, 2012, 11(2):189~209.
19 Saha R, Killian S, Donofrio R S.DNA vaccines:A mini review[J]. Recent Pat DNA Gene Seq, 2011,5(2):92~96.
20 Sautter J, Olesen O F, Bray J, et al. European Union vaccine research——an overview[J]. Vaccine, 2011,29(39):6723~6727.
21 Sloan R D, Wainberg M A.The role of unintegrated DNA in HIV infection[J]. Retrovirology, 2011,8:52.
22 Tang D C, Devit M, Johnston S A. Genetic immunization is a simple method for eliciting an immune response[J].Nature, 1992,356(6365):152~154.
23 Ulmer J B, Donnelly J J, Parker S E, et al. Heterologous protection against influenza by injection of DNA encoding aviral protein[J].Science,1993,259(5102):1745~1749.
24 Wang G, Pan L, Zhang Y.Approaches to improved targeting of DNA vaccines[J]. Hum Vaccin, 2011,7(12):1271~1281.
25 Wang Y, Guo Y, Wang X, et al. Serum amyloid P component facilitates DNA clearance and inhibits plasmid transfection: Implications for human DNA vaccine[J]. Gene Ther, 2012,19(1):70~77.
26 Wolff J A, Malone R W, Williams P, et al. Direct gene transfer into mouse muscle in vivo[J]. Science, 1990,247(4949pt1):1465~1468.

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