生泰素奶牛后药浴液的制备、有效性及安全性研究

doi:10.16431/j.cnki.1671-7236.2023.04.033

摘要/Abstract

摘要： 【目的】研究制备一种新型抗菌肽——生泰素奶牛成膜型后药浴液,对其有效性和安全性进行表征。【方法】运用单因素影响试验及L₉(3)³正交试验筛选生泰素奶牛后药浴液最优配方;运用体外杀菌试验表征杀菌有效性;通过溶血性试验、细胞毒性试验、单次给药小鼠皮肤刺激性试验、多次给药小鼠皮肤刺激性试验及小鼠皮肤致敏性试验验证安全性。【结果】正交试验结果显示,以7%的成膜剂PVA1788为基质配制膜溶液,甘油的试验影响最大,其次为吐温80;生泰素奶牛后药浴液的最优配方为:增塑剂甘油的最优添加体积分数为4%,表面活性剂吐温80的最优添加体积分数为1%,增稠剂普鲁兰多糖的最优添加质量百分浓度为0.4%。生泰素奶牛后药浴液在30 min内可杀灭99.999%以上供试金黄色葡萄球菌、表皮葡萄球菌、无乳链球菌和停乳链球菌,杀菌效果与聚维酮碘相当。生泰素奶牛后药浴液溶血性和细胞毒性均低于聚维酮碘后药浴液。单次和多次小鼠皮肤刺激性试验及皮肤致敏性试验结果显示,生泰素奶牛后药浴液对小鼠皮肤无刺激性和致敏性。【结论】生泰素奶牛后药浴液是一种制备工艺简单、有效、绿色、安全的成膜型后药浴液,预期能有效预防奶牛乳房炎的发生,保证原料乳的质量安全,促进乳品行业健康发展。

关键词: 生泰素; 后药浴液; 正交设计; 有效性; 安全性

Abstract: 【Objective】 The purpose of the experiment was to prepare a new antibacterial peptide-Shengtaicin post-medicated bath solution,and characterize its effectiveness and safety.【Method】 The optimal formulation of Shengtaicin post-medicated bath solution was screened by one-way effect test and L₉(3)³ orthogonal test.In vitro bactericidal test was used to characterize the bactericidal efficacy of the post-medicated bath solution.The safety was verified by hemolysis test,cytotoxicity test,skin irritation test in single-administered mice,skin irritation test in multiple-administered mice and skin sensitization test in mice.【Result】 The orthogonal test results showed that,the film solution was configured with 7% film-forming agent PVA1788 as the substrate,and glycerol had the greatest experimental impact,followed by Tween 80.The optimal formulation of the Shengtaicin post-medicated bath solution was as follow:The optimal addition volume fraction of the plasticizer glycerol was 4%,the optimal addition volume fraction of the surfactant Tween 80 was 1%,and the optimal addition mass percentage concentration of the thickener pullulan polysaccharide was 0.4%.More than 99.999% of the tested Staphylococcus aureus,Staphylococcus epidermidis,Streptococcus agalactiae and Streptococcus dysgalactiae were killed within 30 min.The bactericidal effect was comparable to that of povidone iodine.In addition,the hemolytic and cytotoxic properties of Shengtaicin post-medicated bath solution were lower than those of the post-bath solution of povidone-iodine.The results of single and multiple skin irritation test and skin sensitization test in mice showed that Shengtaicin post-medicated bath solution was non-irritating and non-sensitizing to the skin of mice.【Conclusion】 The antimicrobial peptide Shengtaicin post-medicated bath solution was a validity,green and safe bath,which could effectively prevent the occurrence of mastitis in cows,and it would ensure the quality and safety of raw milk and promote the healthy development of the dairy industry.

Key words: Shengtaicin; post-medicated bath solution; orthogonal design; efficacy; safety

中图分类号:

S858.23

李园园, 杨娜, 滕达, 郝娅, 马炫炫, 毛若雨, 王建华. 生泰素奶牛后药浴液的制备、有效性及安全性研究[J]. 中国畜牧兽医, 2023, 50(4): 1617-1631.

LI Yuanyuan, YANG Na, TENG Da, HAO Ya, MA Xuanxuan, MAO Ruoyu, WANG Jianhua. Preparation and Evaluation of Efficacy and Safety of Shengtaicin Post-medicated Bath Solution for Dairy Cow[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1617-1631.

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