牡荆水提物急性毒性与30 d喂养试验研究

doi:10.16431/j.cnki.1671-7236.2021.06.041

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (6): 2262-2270.doi: 10.16431/j.cnki.1671-7236.2021.06.041

• 基础兽医 • 上一篇

牡荆水提物急性毒性与30 d喂养试验研究

向蓉^1,3, 李亚晖¹, 高原⁴, 魏琦麟¹, 舒柄垚¹, 罗胜军^1,2

1. 广东省农业科学院动物卫生研究所, 农业农村部兽用药物与诊断技术广东科学观测实验站, 广东省畜禽疫病防治研究重点实验室, 广东省中兽药工程技术研究中心, 广州 510640;
2. 广东省前沿动物保健有限公司, 广州 510550;
3. 岭南现代农业科学与技术广东省实验室茂名分中心, 茂名 525000;
4. 珠海市启信达生物科技有限公司, 珠海 519000

收稿日期:2021-01-05 出版日期:2021-06-20 发布日期:2021-06-18
通讯作者: 罗胜军 E-mail:422368881@qq.com
作者简介:向蓉(1981-),女,土家族,湖北来凤人,博士,副研究员,研究方向:兽药,E-mail:23753842@qq.com
基金资助:
广州市科技计划项目（201807010060）；广宁县肉鸽产业园（GDSCYY2020-017）；广东省农业科学院横向项目（2020-DWS-0666）

Study on Acute Toxicity and 30-day Feeding Test of Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz.Water Extract

XIANG Rong^1,3, LI Yahui¹, GAO Yuan⁴, WEI Qilin¹, SHU Bingyao¹, LUO Shengjun^1,2

1. Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Chinese Traditional Medicine Engineering Technology Research Center of Guangdong Province, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Guangdong Qianyan Animal Health Co., Ltd., Guangzhou 510550, China;
3. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China;
4. Zhuhai Qixinda Biotechnology Co., Ltd., Zhuhai 519000, China

Received:2021-01-05 Online:2021-06-20 Published:2021-06-18

摘要/Abstract

摘要： 为探讨牡荆作为中兽药使用的可行性与安全性，本试验对牡荆水提物进行急性毒性试验与30 d喂养试验研究。急性毒性试验采用20只SPF级昆明小鼠，随机均分为药物组和空白对照组，药物组按生药浓度40 mg/g体重进行灌胃，空白对照组灌胃等体积生理盐水，连续观察7 d，结束后重复试验1次；30 d喂养试验采用80只SPF级SD大鼠，随机均分为高（40 mg/g体重）、中（20 mg/g体重）、低（10 mg/g体重）剂量组和空白对照组4个组进行药料饲喂，每组雌雄各半，连续给药30 d，每周称重1次，第30天称重后采血检测血液学指标和血液生化指标，取脏器称重计算脏器系数，高剂量组和空白对照组主要脏器固定后制作切片观察组织病变。结果显示，急性毒性试验中，牡荆水提物的LD₅₀>40 000 mg/kg体重。30 d喂养试验中，大鼠整体状况良好，除高剂量组的体增重极显著低于空白对照组（P<0.01）外，其他各剂量组与空白对照组无差异；高剂量组雄性大鼠脾脏和中剂量组雄性大鼠睾丸脏器系数均显著高于空白对照组（P<0.05），高剂量组雄性大鼠睾丸的脏器系数极显著高于空白对照组（P<0.01），雌性大鼠各剂量组与空白对照组间脏器系数无显著差异（P>0.05）。中剂量组和低剂量组单核细胞数量分别极显著和显著高于空白对照组（P<0.01；P<0.05），其他各剂量组各项血液学指标和血液生化指标与空白对照组之间均无显著差异（P>0.05），且均处于正常范围内。组织病理学检查未发现明显变化。结果表明，本试验条件下牡荆水提物无明显急性毒性和亚急性毒性，安全性较高，可作为一种安全的中兽药进行进一步开发利用。

关键词: 牡荆水提物; 急性毒性; 30 d喂养试验; 安全性

Abstract: To explore the feasibility and safety of Vitex negundo L.var.cannabifolia (Sieb.et Zucc.)Hand. -Mazz.(V.negundo) as a traditional Chinese veterinary medicine,the acute toxicity test and 30-day feeding test were carried out on V.negundo water extract.In the acute toxicity experiment,20 SPF Kunming mice were randomly divided into drug group and blank control group.The drug group was intragastrically administered at a crude drug concentration of 40 mg/g body weight,and the blank control group was intragastrically administered with an equal volume of normal saline for continuous observation for 7 days.Repeat the test once more.The 30-day feeding test used 80 SPF SD rats,which were randomly divided into four groups,high (40 mg/g body weight),medium (20 mg/g body weight) and low (10 mg/g body weight) dose groups,and blank control group,half male and female in each group.Drug feeding were continuously administed for 30 days.Weighed once a week,blood was collected after weighing on the 30th day to test hematological and blood biochemical indicators,organs were weighed to calculate the organ coefficient,the main organs of high-dose group and the blank control group were fixed and then were made slices to observe the tissue lesions.The results showed that LD₅₀ of V.negundo water extract was >40 000 mg/kg in the acute toxicity test.In 30-day feeding test,the overall condition of the rats was good throughout the test period.The body weight of high-dose group was extremely significantly lower than those in blank control group (P<0.01),there was no significant difference in other groups.The organ coefficients of spleen of male rats in the high-dose group and testis of male rats in middle-dose group were significantly higher than that in blank control group (P<0.05),the organ coefficient of testis of male rats in high-dose group was extremely significantly higher than that in blank control group (P<0.01),there was no significant difference in the organ coefficient between the groups of female rats.The number of monocytes in middle-dose and low-dose groups were extremely significantly and significantly higherthan that of blank control group (P<0.01;P<0.05),respectively,there was no significant difference between other hematological indexes and blood biochemical indexes (P>0.05),and they were all within the normal range.Histopathological examination showed no siginificant changes.It showed that V.negundo water extract had no obvious acute and subacute toxicity under the experimental conditions,and had high safety.V.negundo water extract could be used as a safe Chinese veterinary medicine for further development and utilization.

Key words: Vitex negundo L. var.cannabifolia (Sieb.et Zucc.) Hand. -Mazz.water extract; acute toxicity; 30-day feeding test; safety

中图分类号:

S853.7

向蓉, 李亚晖, 高原, 魏琦麟, 舒柄垚, 罗胜军. 牡荆水提物急性毒性与30 d喂养试验研究[J]. 中国畜牧兽医, 2021, 48(6): 2262-2270.

XIANG Rong, LI Yahui, GAO Yuan, WEI Qilin, SHU Bingyao, LUO Shengjun. Study on Acute Toxicity and 30-day Feeding Test of Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz.Water Extract[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2262-2270.

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牡荆水提物急性毒性与30 d喂养试验研究

Study on Acute Toxicity and 30-day Feeding Test of Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz.Water Extract

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